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yes an organism can be MR & VP positive both. for example staphylococcus.

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M-R (Methly Red) is a test used primarily to identify gram-negative rods. M-R is a pH indicator that changes when the pH is below 4.5. The indicator will turn red below 4.5 and yellow, in a pH above that point.

V-P (Voges-Proskauer) is a specific test for metabolic intermediates when sugars ferment to produce butylene glycol. This does occur is some gram negative rods.

A positive V-P test is almost never observed with a positive M-R test. The M-R test is based on the production of sufficient acid to lower the pH to below 4.5. If some of the sugar being fermented is being used to make butylene glycol, then it cannot be used to also produce acids.

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14y ago
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11y ago

Yes. Pseudomonas aeruginosa gives a negative on both tests because it is a glucose non-fermenter.

(I don't know if there are any *other* organisms that do, but it's not impossible)

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14y ago

Nope. bacteria can only be one or the other. MR+ produce acids while VP+ bacteria produce alcohols.

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11y ago

hafnia alvei,proteus mirabilis,serrasia mercescens, protoea agglomerance.

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Q: Could and organism be both MR and V-P positive?
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Could an organism be a fermenter and also be both MR and V-P negative?

Yes, an organism can be a fermenter and be both MR (methyl red) and V-P (Voges-Proskauer) negative. Fermentation is a metabolic process that converts sugar into acids, alcohols, or gases, but the MR and VP tests specifically evaluate different aspects of sugar metabolism that may not be related to fermentation. So, an organism can still ferment sugars without giving positive results on these tests.


What does a positive Voges-Proskauer test mean?

A positive Voges-Proskauer test indicates the presence of acetoin, indicating the fermentation of glucose into a non-acid end product by certain bacteria like Enterobacter and Klebsiella. This test is usually conducted to differentiate between bacteria that can produce acetoin (positive) from those that cannot (negative).


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Related questions

Could an organism be both MR and V-P positive?

No, an organism cannot be both MR (methyl red) positive and VP (Voges-Proskauer) positive. These tests are mutually exclusive and based on different metabolic pathways. An organism either produces acidic byproducts (MR positive) or neutral byproducts (VP positive) from glucose fermentation, but not both.


Could an organism be a fermenter and also be both MR and V-P negative?

Yes, an organism can be a fermenter and be both MR (methyl red) and V-P (Voges-Proskauer) negative. Fermentation is a metabolic process that converts sugar into acids, alcohols, or gases, but the MR and VP tests specifically evaluate different aspects of sugar metabolism that may not be related to fermentation. So, an organism can still ferment sugars without giving positive results on these tests.


Organism which is indole negative mr positive vp negative citrate positive?

Sounds like Citrobacter freundii. Double check that you do have a gram negative rod that is motile, H2S negative and oxidative negative (most gram neg rods are).


What does a positive Voges-Proskauer test mean?

A positive Voges-Proskauer test indicates the presence of acetoin, indicating the fermentation of glucose into a non-acid end product by certain bacteria like Enterobacter and Klebsiella. This test is usually conducted to differentiate between bacteria that can produce acetoin (positive) from those that cannot (negative).


Organism which is citrate positive indole negative MR negative VP negative sorbitol negative?

The organism is likely to be Escherichia coli. E. coli is citrate positive since it can utilize citrate as a carbon source, but it is indole negative as it does not produce indole from tryptophan. Additionally, E. coli is MR (methyl red) negative and VP (Voges-Proskauer) negative, indicating its fermentation pattern. Lastly, E. coli is typically sorbitol negative, meaning it does not ferment sorbitol.


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Why is A positive VP test is almost never observed with a positive MR test?

The VP (Voges-Proskauer) test and the MR (methyl red) test detect different fermentation products in bacteria. A positive VP test indicates the production of acetoin, while a positive MR test indicates the production of mixed acids. Since the pathways for producing these two products are often mutually exclusive in bacteria, it is rare to observe a positive result in both tests simultaneously.


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