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The retention time would increase becasue longer distance would be travelled by the analyte!

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14y ago
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wonderpush

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2y ago

When the column length is doubled, the retention time is also doubled.

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Q: Are there any advantages in increasing retention time?
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Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc


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