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I would not recommend it as the flea killing agent is still on the skin. But you do have to get rid of those fleas on her. Give her a flea bath and then re wash the breast area with a good dog shampoo but not one meant to kill fleas & rinse very well. Check out the pups carefully if the Mom has fleas as they probably have them too. Take all the bedding out of her whelping box and wash thoroughly. You can spray some flea spray onto your hands lightly and then rub that onto the pups skin, keeping it away from their eyes. I recommend you spray the flea spray on your hands then wipe a circle around their necks first with your hands to try to prevent the fleas from running to the head area.

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Q: Can deflea bath a female dog while lacting puppies?
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