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Animal Models of Diabetic Complications Consortium AMDCC Protocols Low-Dose Streptozotocin Induction Protocol (Mouse)

Version: 1

Replaced by version: N/A

Edited by: The University of Michigan Medical Center , Frank Brosius

Summary

Reagents and Materials

Protocol

Reagent Preparation

Na-Citrate Buffer

Streptozotocin (STZ)

STZ-Na-Citrate Solution

Summary: This protocol is used by AMDCC members to induce Diabetes in a number of the animal models developed by the consortium. This induction protocol is used to induce a diabetes similar to a type I diabetic.

Reagents and Materials: Reagent/Material

Quantity Required

Vendor

Stock Number

STZ-Na-Citrate Solution

See below

TB or insulin syringes w/ needle attached (26-28 ½ gauge)

As many as necessary for the number of mice

Isoflurane drop jar

Isoflurane

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Q: How do you prepare streptozotocin solution for the induction of diabetes?
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