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Version: 1
Replaced by version: N/A
Edited by: The University of Michigan Medical Center , Frank Brosius
Summary
Reagents and Materials
Protocol
Reagent Preparation
Na-Citrate Buffer
Streptozotocin (STZ)
STZ-Na-Citrate Solution
Summary: This protocol is used by AMDCC members to induce Diabetes in a number of the animal models developed by the consortium. This induction protocol is used to induce a diabetes similar to a type I diabetic.
Reagents and Materials: Reagent/Material
Quantity Required
Vendor
Stock Number
STZ-Na-Citrate Solution
See below
TB or insulin syringes w/ needle attached (26-28 ½ gauge)
As many as necessary for the number of mice
Isoflurane drop jar
Isoflurane
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Is citrate buffer sterile for making Streptozotocin?
how to prepare citrate buffer for making Streptozotocin
How prepare an induction report?
To prepare an induction report, there are several key factors to consider. What's in the report, can't pass or fail the induction, keep progress notes, set an assessment meeting date, check that boxes are appropriately ticked, proactive editing, and always keep a copy.
How can prepare .01N KBr solution?
To prepare a 0.01N KBr solution, dissolve 0.74g of KBr in 1 liter of water. This will give you a solution with a molarity of 0.01N for KBr.
How do you prepare 30 percent sucrose solution?
See the two Related Questions to the left for the answer.The first is how to prepare a solution starting with a solid substance (and dissolving it). The second question is how to prepare a solution by diluting another solution.
How do you prepare buffer solution?
To prepare a buffer solution which may be acidic. Titrate ethanoic acid (weak acid) with sodium ethanoate(salt).
How do you prepare 05M glutaraldehyde?
To prepare a 0.5M glutaraldehyde solution, you would need to dilute a concentrated glutaraldehyde stock solution with the appropriate volume of water or buffer solution. Calculate the volume of stock solution needed based on the desired final volume and concentration, then dilute with the solvent. Finally, mix the solution thoroughly to ensure uniform distribution. Remember to follow safety protocols when working with glutaraldehyde, as it is a hazardous chemical.
How can a solution of ammonia be used to prepare ammonia sulphate?
To prepare ammonium sulfate from a solution of ammonia, you would react the ammonia solution with sulfuric acid. The chemical equation for this reaction is: 2NH3 + H2SO4 → (NH4)2SO4. This reaction will result in the formation of ammonium sulfate, which can then be isolated by evaporating the water.
How can you prepare 50mm glucose?
To prepare a 50mm glucose solution, you would need to dissolve 9g of glucose in enough water to make 100mL of solution. This would give you a solution with a concentration of 50mm (millimolar).
How do you prepare 2 ppm solution of nickel nitrate?
To prepare a 2 ppm solution of nickel nitrate, you would dissolve 2 grams of nickel nitrate in enough water to make 1 liter of solution. This will result in a solution where there are 2 parts of nickel nitrate for every 1 million parts of water.
Is it possible to prepare 0.1m solution of 0.01M solution?
Yes, by décreasing the volume tenfold.
What is the composition of gowers solution?
how do you prepare gowers solution the red blood cells diluting fliud
How do you prepare ascorbic acid solution?
To prepare an ascorbic acid solution, dissolve the appropriate amount of ascorbic acid powder in distilled water. Stir the solution until the powder is completely dissolved. Ensure the solution is properly mixed and then store it in a clean container.
