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to remove tannins and polyphenols which are readily present in crude plant extracts

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Q: What is the role of beta mercaptoethanol in DNA extraction?
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What is the role of beta-mercaptoethanol in DNA extraction?

stabilization of phenol against oxidation


What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?

It is an antioxidant.


What is the role of NaCl in DNA precipitation?

roll of Na CL in DNA extraction


What is the Role of glycerol in DNA extraction?

Glycerol is sometimes added to DNA extraction buffers to increase the density of the solution, allowing DNA to precipitate more efficiently. It also helps stabilize DNA during extraction procedures by preventing degradation from nucleases.


Role of tris in extraction of DNA?

To give the solution buffering capacity.


What role does EDTA play in DNA extraction processes?

EDTA is a chelating agent that helps to bind and remove metal ions that can degrade DNA during extraction processes. It helps to stabilize the DNA and prevent enzymatic degradation, allowing for a more efficient and successful extraction of DNA.


Role of beta-mecaptoethanol in DNA isolation?

beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.


What is the role of mercaptoethanol in DNA isolation?

Mercapotoethanol is used as an antioxidant. Especially for polyphenolics which become insoluble when oxidised and tend to precipitate with the DNA. Sodium sulphite (1.2 g/L) does the same thing without the stink.


What is the Role of calcium acetate in DNA extraction?

Calcium acetate is used in DNA extraction to neutralize the negative charge of DNA molecules, allowing them to aggregate and precipitate out of solution. This helps to separate DNA from other cellular components during the extraction process, making it easier to isolate pure DNA for downstream applications.


What is the role of 70 ethanol in DNA extraction?

70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.


What is the role of isopropanol in DNA extraction?

Isopropanol is used in DNA extraction to separate DNA from other cellular components. It helps to precipitate the DNA, causing it to clump together and separate from the rest of the solution. This allows for the isolation and purification of the DNA for further analysis.


What is the role of ethanole in DNA isolation?

2-Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract. It may also help to denature proteins by breaking disulphide bonds between cysteine residues.