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to remove tannins and polyphenols which are readily present in crude plant extracts
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∙ 11y ago
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What is DNA medicine?
DNA has largely replaced finger-prints for indentifying people. Or at least confirmed what finger-prints have led authorities to. Service people in the military today, all must now submit their DNA to the medical corps, so that in the event they become a casualty, they can be identified. DNA science has probably eliminated future "Unknown Soldiers." For detailed definitions of DNA, see site: DNA
What key event happens during the s phase?
synthesis of DNA DNA Replication
Are genes a length of DNA?
Yes, a gene is a complex length of DNA that is in our body. A gene is a length of DNA that codes for a living life form. Genes are found on chromosomes which are in the body nucleus.
Did an African American contribute to the discovery of DNA?
No, DNA was first isolated by the Swiss physician. Also there were no African Americans involved in any subsequent work on DNA leading to the understanding of it structure.
When does DNA occur in the cell cycle?
All of it.The cell cycle describes the state of the DNA in the cell.
What is the role of beta-mercaptoethanol in DNA extraction?
stabilization of phenol against oxidation
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is the role of NaCl in DNA precipitation?
roll of Na CL in DNA extraction
What is th role of dnazol in dna extraction?
DNAzol is a reagent used in DNA extraction to lyse cells by disrupting the cell membrane and nucleus. It helps release DNA from the cells and proteins, allowing for subsequent separation and purification of the DNA. DNAzol also helps protect the DNA from degradation during the extraction process.
Role of tris in extraction of DNA?
To give the solution buffering capacity.
What is the role of hot water bath in DNA extraction?
The hot water bath is used in DNA extraction to break down cell membranes and release the DNA. By placing the sample in a hot water bath, the heat helps to disrupt the cell structure, releasing the DNA from the cells. This process is key in isolating the DNA for further analysis.
What is the role of mercaptoethanol in DNA isolation?
Mercapotoethanol is used as an antioxidant. Especially for polyphenolics which become insoluble when oxidised and tend to precipitate with the DNA. Sodium sulphite (1.2 g/L) does the same thing without the stink.
Role of beta-mecaptoethanol in DNA isolation?
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.
What is the role of ethanole in DNA isolation?
2-Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract. It may also help to denature proteins by breaking disulphide bonds between cysteine residues.
What is the role of 70 ethanol in DNA extraction?
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
What role does a cheese cloth play in DNA extraction?
A cheese cloth is typically used in DNA extraction to filter out solid cell debris from the sample, leaving behind only the liquid DNA-containing solution. This helps to ensure a cleaner and more purified DNA extract that can be further processed and analyzed.
What is the role of heat in DNA extraction?
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
