No. Father, Mother, siblings and children are immediate relatives.
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Yes, your husband is considered to be your closest relative.
In English there are no masculine or feminine forms. English uses gender specific nouns for male or female.The noun 'uncle' is a singularnoun for a male relative.(Although your uncle himself is, probably, masculine.)The corresponding singular noun for a female relative is aunt.
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That's actually determined by state law. But usually it would be the closest blood relative an adult sibling if applicable, followed by maternal grandparent(s), paternal grandparent(s), aunt or uncle, and so forth.
Your uncle's wife is your aunt.