The lower transmittance of film would indicate a higher Optical Density (OD), or absorption, because as concentration increases, % transmittance will decrease. This is because of Beer's Law (A = 2.00 -[log (%T)]). As you can see from the equation, transmittance is directly proportional to the outcome of absorption. High %T = Lower Absorption.Low %T = Higher Absorption.
how do you convert percent transmittance to mg/l
The difference in percentage of transmittance between live chloroplasts incubated in light vs. dark may be due to the light-dependent reactions of photosynthesis. In the light, chloroplasts undergo photosynthesis, leading to the absorption of light energy and production of oxygen, which can affect the transmittance. In contrast, chloroplasts kept in the dark are not actively performing photosynthesis, resulting in different levels of pigment activation and physical changes that can influence transmittance measurements.
There is none.
034 is less than 75.
A=logIo/I
034 is an integer, not a fraction and it makes little sense to convert it to a fraction. However, if you must, it is simply 034/1 = 34/1.
effect of solvent on UV-Visible spectrum
No, light transmittance cannot be more than 100 percent. Transmittance is a measure of how much light passes through a material or substance, and it is typically expressed as a percentage between 0% and 100%. If light transmittance were to exceed 100%, it would imply that more light is transmitted than actually strikes the material, which is not physically possible.
Absorbance measures the amount of light absorbed by a sample, while transmittance measures the amount of light that passes through a sample. Absorbance is calculated as -log(T), where T is transmittance. Absorbance is commonly used in spectrophotometry to quantify the concentration of a substance in a solution.
Color and transmittance are related because the color of an object is influenced by the wavelengths of light it absorbs and reflects. When light passes through a material, the transmittance of that material refers to how much of the light is able to pass through. The color of the transmitted light may be altered depending on which wavelengths are being absorbed or transmitted.
Absorbance = -log (percent transmittance/100)