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Normally no.
While brain cells can die off after as little as 3 minutes without oxygen; under normal conditions your body will force you to breath before you hit the point where brain cells begin to die.
The only way you could force yourself to lose brain cells from holding your breath would be to add additional complications that prevent the bodies natural danger response from saving you.
In other words: if you add a plastic bag covering the mouth, or hold your breath under water or use some other extreme method of blocking new air flow than yes it would be possible to kill brain cells; however, at that point you are no longer actually "holding your breath" your body would be trying to breath but you would be depriving it of oxygen through additional methods.
You will have at least attempted to breath before any brain cells will die, the only question at that point is if there is air available for you to breath or are you immersed in water, plastic or something else which prevents it from happening.
Even in the most extreme cases of breath holding, the most you could do is force yourself to pass out, at which point your body will begin breathing and prevent brain damage; unless of course you have covered your air passages in some other way.
Huffing, in the majority of cases, induces its effects by creating a condition of partial suffocation. When cells don't get enough oxygen, they die -- especially brain cells, which die even faster than most.
Have fun!
On the contrary. The brain needs three things to survive: blood, oxygene and glucose. You must have noticed that when you are tired or sleepy and you go outside to get some fresh air you wake up. That's because more oxygen is sent to the brain.
No, it doesn't. Some people think so, but it does skip a heart beat.
I hope you found this helpful.
Dramatically over sleeping can weaken brain cells, and cause them to be less efficient.
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