A recombinant sequence of DNA is a sequence of DNA that comes from more than one source. Examples of recombinant DNA are plasmids that are put into bacteria. The plasmid comes from the bacteria (or a bacteria at least) but a target gene has been added (say the lac operon gene that allows bacteria to thrive on lactose), this plasmid is now a recombinant DNA sequence.
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to differenciate recombinant dna from non recombinant
recombine joins together with means that the plasmid and the foreign dna join together to make recombinant dna
Ni-NTA column is a nickel-nitriloacetic acid column that is used to bind polyhistidine residues in order to purify recombinant proteins. The polyhistidine sequence (six histidines in a row) will bind to nickel, which is in turn bound by the nitroloacetic acid. This immobilizes the recombinant protein in the column for later extraction. Polyhistidine-tagging and Ni-NTA column purification:http://en.wikipedia.org/wiki/Polyhistidine-tagNi-NTA product:http://www.biocompare.com/Articles/ProductReview/825/ProductReview.html
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