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The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one

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Edyth Anderson

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3y ago
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11y ago

A pure culture is the study of the properties of one particular microbe eliminating extraneaous influences. (without contamination)


Simply put, a pure culture is defined as a growth of a microorganism containing only one cell type type, i.e. only a single species
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Q: What is a pure culture?
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Why obtain pure culture of bacteria from a mixed culture?

i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture


Is pure culture having same meaning of microbial culture?

Yes, a pure culture refers to a microorganism culture that contains only a single strain or species, free from contamination. Microbial culture is a broader term that can refer to any type of culture of microorganisms, including pure cultures or mixed cultures containing multiple strains or species.


What is the difference between pure culture and auxanic culture?

A pure culture contains only one type of microorganism, while an auxanic culture is a type of pure culture that has been enriched to promote growth of specific microorganisms, typically for research purposes. Auxanic cultures may contain growth factors or nutrients that allow the targeted microorganism to thrive.


Could you distinguish between axenic culture and pure culture?

An axenic culture refers to a culture containing only one species of microorganism without any other contaminants present. A pure culture, on the other hand, refers to a culture containing only one specific strain or species of microorganism, but it may still have contaminants present. In essence, axenic culture is free of any contaminants, while a pure culture may have contaminants other than the desired microorganism.


How would you define pure culture sterile and contamination in the isolation and purification of bacteria?

A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.


How would you define pure culture and sterile and contamination in the isolation and purification of bacteria?

A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.


Which culture medium preparation is most commonly utilized in isolation of pure culture?

Solid media is used for the isolation of bacteria as pure culture. 'Agar' is most commonly used to prepare solid media.


Did you get a pure culture in your subculture and how to know?

To confirm if you have a pure culture in your subculture, you can streak the culture on a fresh agar plate and observe if only one type of colony grows. If multiple colony types appear, it suggests contamination, and the culture is not pure. Additionally, conducting Gram staining or molecular techniques can further confirm purity by identifying the specific microorganism present.


What is a bacterial culture containing a single species of organisms referred to as?

A bacterial culture containing a single species of organisms is referred to as a pure culture. This means that there is only one type of bacteria present in that culture without any contamination from other species. Pure cultures are essential for studying specific bacterial characteristics and behavior.


Why media culture is specifically?

Culture media should be specific to avoid contamination of unwanted microorganisms and to obtain a pure culture of microorganisms.


What type of culture technique would be used to obtain a pure culture?

To obtain a pure culture, a technique called streak plate method is commonly used. This technique involves streaking a sample on an agar plate in a way that isolates individual colonies, allowing for the growth of pure cultures. Subsequent subculturing from a single isolated colony can help to ensure a pure culture.


What are the materials required to perform pure culture?

To perform a pure culture, you will need culture media (agar plates or broth), inoculation loop or needle, incubator, and the sample containing the microorganism you want to culture. These materials will help create a controlled environment for the growth of a single species of microorganism.