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I think you mean electrphoresis buffer ( anyway the principle is the same for all sloutions,, simply it is chemistry ) if so , you'll need to take about 25 ml from your 40X buffer and complete them till 1000 ml with distilled water.

The calculation can be done as the following N * V = N' * V'

so 40X * Z = 1X * 1000 Z is the amount of 40X buffer needed to be diluted to give us 1L of 1X buffer

so Z = 1000/40=25 ml

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Q: How do you convert 40X gel buffer into 1X buffer?
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