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The buffer capacity increases as the concentration of the buffer solution increases and is a maximum when the pH is equal to the same value as the pKa of the weak acid in the buffer. A buffer solution is a good buffer in the pH range that is + or - 1 pH unit of the pKa. Beyond that, buffering capacity is minimal.
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How did binding buffer get its name?
A binding buffer is a substance used in chromatography to fix a specific compound.For example this buffer can be linked to a protein.
What is the full name for te buffer?
It is a buffer used in biology. "te" is derived from its components: t from tris, a common pH buffer, and e from the EDTA, a molecule. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Industrial applications of common ion effect?
buffer solutions are the use ful applications of common ion effect they are important for biological applications[some enzymes can only work at a specific ph,the ph of gastric juices is 1.5. chemical applications fermentations,dyeing need a maximum ph.
What is the purpose of buffer?
Buffer Resist and Maintains the PH of the solution if there change in the environment of the solution.
In this procedure a 10x buffer is used if you have a 5x buffer and you want a total volume of 200ul what dilution would you perform to achieve the desired 1x concentration of buffer?
10x to 1x is a 1:10 dilution Therefore, add 1 part buffer, 9 parts DI-water If 100uL is 10uL (1 part buffer) and 90uL (9 parts DI-water) Then, 200ul (100 x 2) is 20uL (1 part buffer) and 180uL (9 parts DI-water)
