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STUDY OF QUANTITY OF CAESIN PRESENT IN DIFFERENT SAMPLES OF MILK
A FILE REPORT SUBMITTED TO
INTERNAL
EXAMINER
CHEMISTRY DEPARTMENT ADITYA Public School Ghaziabad U.P
CERTIFICATE
This is to certify that this dissertation titled "STUDY OF QUANTITY OF CAESIN PRESENT IN DIFFERENT SAMPLES OF MILK" submitted by
Arun Bhardwaj to Chemistry department of THE, was ADITYA Public School Ghaziabad U.P
carried under guidance and supervision during the academic year 2011-2012.
Principal Mr………………
BACKNOWLEDGEMENT
I wish to express my deep gratitude and sincere thanks to the Principal, ADITYA Public School Ghaziabad U.P for her encouragement and for all the facilities that she provided for this project work. I sincerely appreciate this magnanimity by taking me into her fold for which I shall remain indebted to her. I extend my hearty thanks to Mr. …………….chemistry teacher, who guided me to the successful completion of this project. I take this opportunity to express my deep sense of gratitude for his invaluable guidance, constant encouragement, constructive comments, sympathetic attitude and immense motivation, which has sustained my efforts at all stages of this project work.
I can't forget to offer my sincere thanks to Mr. ………………… & also to my classmates who helped me to carry out this project work successfully & for their valuable advice & support, which I received from them time to time.
Arun bhardwaj
DEDICATION
I DEDICATE THIS PROJECT WORK TO THE LOTUS FEET OF
MY FATHER Mr. Om prakash & MY MOTHER Mrs. Mrs Usha
DECLARATION
I do hereby declare that this project work has been originally carried under
the guidance and supervision of Mr…………………………………………., head of chemistry
department, ADITYA Public School Ghaziabad U.P
Arun Bhardwaj
INDEX
1. Introduction
2. Aim
3. Requirements
4. Theory
5. Procedure
6. Observations
7. Conclusions
8. References
Introduction
Milk is a complete diet as it contains in its Minerals, Vitamins Proteins, Carbohydrates, Fats And Water. Average composition of milk from different sources is given below:
Source
Water
Mineral
Protei
Fats
Carbohydra
of milk
(%)
s (%)
ns(%)
(%)
tes (%)
Cow
87.1
0.7
3.4
3.9
4.9
Human
87.4
0.2
1.4
4.0
4.9
Goat
87.0
0.7
3.3
4.2
4.8
Sheep
82.6
0.9
5.5
6.5
4.5
Caesin is a major protein constituent in milk & is a mixed phosphor-protein. Casein has isoelectric pH of about 4.7 and can be easily separated around this isoelectric pH. It readily dissolves in dilute acids and alkalies. Casein is present in milk as calcium caseinate in the form of micelles. These micelles have negative charge and on adding acid to milk the negative charges are neutralized.
Ca2+-Caesinate +
2CH3COOH(aq)^Caesin+(CH3COO)2Ca
AIM
To study the quantity of Casein in different samples of milk.
REQUIREMENTS
> Beakers (250 ml)
> Filter-paper
> Glass rod
> Weight box
> Filtration flask
> Buchner funnel
> Test tubes
> Porcelain dish
> Different samples of milk
> 1 % acetic acid solution
> Ammonium sulphate solution
Theory
Natural milk is an opaque white fluid Secreted by the mammary glands ofFemale mammal . The main constituents of natural milk are Protein, Carbohydrate, Mineral Vitamins,Fats and Water and is a complete balanced diet . Fresh milk is sweetish in taste. However , when it is kept for long time at a temperature of 5 degree it become sour because of bacteria present in air . These bacteria convert lactose of milk into lactic acid which is sour in taste. In acidic condition casein of milk starts separating out as a precipitate. When the acidity in milk is sufficient and temperature is around 36 degree, it forms semi-solid mass, called curd.
PROCEDURE
1. A clean dry beaker has been taken, followed by putting 20 ml of cow's milk into it and adding 20 ml of saturated ammonium sulphate solution slowly and with stirring. Fat along with Caesin was precipitate out.
2. The solution was filtered and transferred the precipitates in another beaker. Added about 30 ml of water to the precipitate. Only Caesin dissolves in water forming milky solution leaving fat undissolved.
3. The milky solution was heated to about 40oC and add 1% acetic acid solution drop-wise, when casein got precipitated.
1. 4. Filtered the precipitate, washed with water and the precipitate was allowed to dry.
2. 5. Weighed the dry solid mass in a previously weighed watch glass.
3. 6. The experiment was repeated with other samples of milk.
OBSERVATIONS
CONCLUSION
Different samples of milk contain different percentage of Caesin.
Casein is a type of protein that makes up 80% of cows milk, and a significant amount of other mammalian milk. To determine the casein in a sample of milk, a person could collect several samples from different cows or other mammals, segregate the casein, and measure it.
add 20ml of milk.in it add 10ml of water........add acetic acid in the milk..precipitate will be formed......that is casein....filter the precipitate and weight it and you'll get the desired.....
The amount of casein in milk before dilution is in mg form - 50.2 102.3 149.6 mg. The amount of casein in milk is about 80% of the milk's protein content.
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