Hexane is a non-polar solvent. Fats are also non-polar substances. Fats are thus highly and rapidly soluble in hexane as compared to any other solvent. Therefore, if a total fat content determination is required, hexane is the best while not suitable for polar and partially polar lipids. For determination of total lipids including all polar, partially polar and non-polar fats, the best described system is Chlroform:Methanol (3:1).
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