Scene 2 Act 2 line 170 Polonius leaves at line 215
"Where is your father?" (said to Ophelia)
Polonius is interested in seeing whether spying on Hamlet's conversation with Gertrude will justify his theory that Hamlet is mad for love. (see the end of Act 3 Scene 1)
The poisoned tip of Laertes' blade cuts Hamlet in their duel in Act V; thus Laertes is the direct cause of Hamlet's death.
Polonius in Act III. Hamlet's father, the Ghost, is already dead when the play starts.
A. Gertrude---------Queen of Denmark B. Polonius----------Murdered by Hamlet C. Ophelia-----------Daughter of Polonius D. Claudius----------King of Denmark
Rosencrantz and Guildenstern spy on Hamlet, Claudius and Polonius spy on Hamlet while he is talking to Ophelia, and Polonius spies on Hamlet when he is talking to Gertrude. On the other hand, Hamlet and Horatio spy on Claudius during the play-within-a-play. And in a completely unrelated bit of spying Polonius gets Reynaldo to spy on Laertes.
Hamlet has his first run-in with Polonius in Act 2 Scene 2. He calls Polonius a fishmonger, a seller of fish, which is slang for a pimp. When Polonius asks, "What is the matter?", meaning "What is the book about?" Hamlet intentionally misunderstands him as meaning "What is the problem?" in the sense of an issue between two people, and says "Between who?"
The ghost, in Act 1 Scene 5.
Polonius is sure that Hamlet has gone mad with love for Ophelia. See these lines by Polonius in Act 2 scene 1: ~Polonius: Come, go with me! I will go seek the King;This is the very ecstasy of love,...Ophelia: ... I did repel his letters, and denied His access to me.Polonius: That hath made him mad;~ So Polonius thinks Hamlet is suffering from the "ecstasy of love" for Ophelia, which has driven him mad.
In Act 3 scene 1 of Hamlet (the "nunnery" scene), Claudius and Polonius eavesdrop on the conversation between Hamlet and Ophelia. Well, it's sort of eavesdropping since Ophelia knows they are there and if Hamlet does not know at the beginning of the conversation, he figures it out pretty quickly.
Hamlet gives his "To be, or not to be" soliloquy. Hamlet tells Ophelia, "Get thee to a nunnery!" Hamlet decides not to kill Claudius while he prays. Hamlet kills Polonius.