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Role of Taq polymerase in PCR?
Taq polymerase is a heat-stable DNA polymerase enzyme that is crucial in polymerase chain reaction (PCR). It is used to amplify DNA by synthesizing new DNA strands complementary to a template DNA strand at high temperatures. Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, allowing it to withstand the high temperatures used in PCR without becoming denatured.
Why taq polymerase is thermostable?
Taq polymerase is thermostable because it comes from a bacterium called Thermus aquaticus, which lives in hot springs and has adapted to high temperatures. The enzyme's structure is able to resist denaturation at high temperatures, allowing it to function optimally in PCR reactions that require high temperatures for DNA amplification.
How did Taq DNA polymerase acquire its name?
The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin
Why taq polymerase is strored at very low temperature as it is thermostable?
Storing Taq polymerase at a very low temperature (typically -20°C) helps preserve its activity over time. While Taq polymerase is thermostable and can withstand high temperatures during PCR, storing it at low temperatures helps prevent degradation and denaturation of the enzyme, leading to better performance in PCR reactions.
What is the source of the polymerase used in polymerase chain reaction?
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
Who is the discoverer of Taq DNA polymerase?
Thomas D. Brock, An American Microbiologist
Why PCR need gelatin?
According to Roche website, different additives allow optimization to increase yield and specificity of PCR reactions. DMSO, for instance, is reported to reduce nonspecific priming, while gelatin and glycerol stabilize Taq DNA polymerase during PCR, which generally increases the yield.
What discovered tag polymerase?
Tag polymerase, also known as Taq polymerase, was discovered in 1976 by researchers at Cetus Corporation. Taq polymerase is a heat-resistant enzyme that is commonly used in polymerase chain reaction (PCR) due to its ability to withstand high temperatures required for DNA amplification. This discovery revolutionized molecular biology research by enabling the automation and rapid amplification of DNA sequences.
Why is a thermostable polymerase used in PCR?
A thermostable polymerase, like Taq polymerase, is used in PCR because it can withstand the high temperatures required for DNA denaturation during each cycle of amplification. This allows the enzyme to remain active throughout the entire PCR process, resulting in efficient and accurate DNA replication.
What is MgCl used for in the polymerase chain reaction?
used as a co-factor o th enzime taq polimerase
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
What is the common name for Thermus aquaticus?
The common name for Thermus aquaticus is "Taq polymerase," which is a heat-resistant enzyme used in polymerase chain reaction (PCR) techniques.
