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In the Indirect ELISA ,

  1. An antigen is added to the microtiter plate well and the antigen attaches to the walls of the microtiter plate.
  2. After rinsing to remove excess antigen, the serum suspected of containing the antibodies is added.
  3. Enzyme-linked antibody capable of reacting with the constant region of other antibodies is the added, followed by addition of the colorless substrate. Development of color indicates the presence of the antibody being identified.
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13y ago
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10y ago

The ELISA (enzyme-linked immunosorbent assay) is a test which uses color change and antibodies to identify a substance. An indirect ELISA is one of three ELISA tests.

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Q: What is indirect elisa?
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What is the difference between direct and indirect ELISA?

In direct ELISA, the primary antibody is directly linked to an enzyme for detection, while in indirect ELISA, a secondary antibody linked to an enzyme is used to detect the primary antibody bound to the antigen. Direct ELISA is quicker and more straightforward, but indirect ELISA allows for signal amplification and detection of multiple antibodies bound to the antigen.


What is ELISA testing?

ELISA is an acronym for Enzyme Linked ImmunoSorbent Assay and is used in a wide variety of applications, including detecting antibodies from HIV. See the related link for more information.(Answer by Syama S.):ELISA is a technique used to determine the presence of antigen or antibody in a sample. ELISA is used in diagnosis of HIV... ELISA is of three types: direct method, indirect method and sandwich method. The principle of three methods are same.ELISA is a technique used to determine the presence of antigen or antibody in a sample. ELISA is used in diagnosis of HIV... ELISA is of three types: direct method, indirect method and sandwich method. The principle of three methods are same.


In indirect elisa the enzyme liked antibody will attach to?

In an indirect ELISA, the enzyme-linked antibody attaches to the target antigen that has been immobilized on the microplate. This allows for the detection of the antigen through the enzyme's activity, which produces a signal that indicates the presence of the target antigen in the sample.


What is Elisa used for?

Elisa (Enzyme-Linked Immunosorbent Assay) is a common laboratory technique used to detect the presence of antibodies or antigens in a sample. It is widely used in medical diagnostics, food testing, and research settings to identify and quantify specific molecules.


When was Elisa's created?

Elisa's was created in 2009.


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