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it is a labours process

it is timeconsuming process

it required more amount of dna require 3microliter dna per sampel

it is costly

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βˆ™ 11y ago
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βˆ™ 14y ago

to get expressed proteins.

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Q: What are the disadvantages of Southern blotting?
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What are some other techniques that build on the Southern Blotting technique?

Some techniques that build on Southern blotting include Northern blotting for RNA detection, Western blotting for protein detection, and Southwestern blotting for DNA-binding proteins detection. These techniques are adaptations of Southern blotting and are used to detect specific molecules in biological samples.


What is the meaning of blotting technique?

Blotting technique is a laboratory method used to transfer biomolecules, such as DNA, RNA, or proteins, from a gel matrix to a membrane for further analysis. There are different types of blotting techniques including Southern blotting for DNA, Northern blotting for RNA, and Western blotting for proteins.


Difference between southern and northern blotting?

Southern blotting is used to detect specific DNA sequences, while northern blotting is used to detect specific RNA sequences. Southern blotting involves DNA samples being digested with restriction enzymes, separated by gel electrophoresis, and then transferred to a membrane for probing. Northern blotting involves RNA samples being separated by gel electrophoresis, transferred to a membrane, and then probed with a complementary RNA or DNA sequence.


Is the principle of northern southern and western blotting is same?

yes the principle of all the three is same .the basic difference lies during probing nd detection .in case of southern blotting DNA is used in nothern rna and in case of western blotting antibodies are ued


Which describes the transfer of mRNAs to a membrane to analyze gene expression?

southern blotting


What is blotting and types of blotting?

Blotting is a technique used to identify specific molecules in a sample by separating them based on size and charge. There are different types of blotting methods, including Southern blotting (detecting DNA sequences), Northern blotting (detecting RNA sequences), and Western blotting (detecting proteins). Each method involves transferring the separated molecules from a gel to a membrane for detection using specific probes or antibodies.


Is etbr transfer with DNA during southern blotting?

Yes, ethidium bromide (EtBr) is commonly used in agarose gel electrophoresis to visualize DNA. However, in Southern blotting, the DNA is transferred from the gel to a membrane (typically a nitrocellulose or nylon membrane) through capillary action or by vacuum or electric transfer methods. EtBr is not usually involved in this transfer process, but rather in visualizing the DNA after the transfer step.


What is a blad?

A blad is a Scots term for a portfolio, blotting-book or blotting-pad.


Can chlamydia cause blotting?

Blotting? As In Bleeding, then yeah It doesYou can bleed from being infected by chlamydia.


What is blotting technique?

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How do you do western blotting from RNA?

A western blot cannot be prepared from RNA. Only a protein sample can be run with a western blotting approach. The technique used to visualize RNA on a gel is called northern blotting.


What is were the advantages and disadvantages of building more towns in the southern colonies?

Colonists