Isolation often leads to speciation, because as each isolated population evolves new characteristics, the separate populations eventually get DNA that is too different for the two to breed and have fertile offspring (this is the point when speciation has occurred). In the case that there is not isolation, the whole species must slowly evolve until it becomes a new species. However, here the line between where the speciation actually occurred becomes blurry, because it doesn't happen in a single generation.
By sympatric and allopatric speciation.
Sympatric speciation is when a new species arises in populations that overlap geographically. This one is rare and more common in plants than animals.
Allopatric speciation is when a new species arises in populations that are separated geographically. This one is more common.
Species (phylogenetically and genetically distinct animals from a common ancestor) form when barriers exist to prevent outbreeding. These are usually environmental (e.g. mountain ranges, oceans, climatic barriers) or biological (e.g. interbreeding of two species results in an infertile offspring).
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There are no discrete steps to speciation. There are a number of boundary conditions that are met in most cases. Speciation is the result of any situation that leads to the genetic, behavioural and/or morphological divergence of subpopulations of the same species, which may in turn lead to a decline in interbreeding frequency. The causes for such divergence may vary. The clearest example of the principle is when two subpopulations become geographically isolated (for instance due to migration, floods or some other natural event), and diverge as a result of genetic drift. Once the two population have diverged to the degree that they would no longer produce fertile offspring together even if they were put back together again, we say that speciation has occurred.
Fat Girl died 3 weeks ago after an intense battle with her psychology leading to her being institutionalized and starting a chain of events that would lead to her suicide.
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