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its used as its a chaotropic salt..dat is it melts the agarose piece containing the DNA fragment when incubated at 45-55 degress Celsius

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8y ago
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4mo ago

Sodium iodide is used for DNA elution because it helps to lower the melting temperature of double-stranded DNA, allowing it to denature and release from the solid support. The high ionic strength of sodium iodide also promotes DNA interactions with the solvent, facilitating elution. Additionally, sodium iodide helps to minimize secondary structure formation by stabilizing the denatured DNA during elution.

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Q: Why sodium iodide for DNA elution?
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Related questions

What is the Ionic Compound of NaI?

The ionic compound of sodium iodide is NaI. It is composed of sodium (Na+) ions and iodide (I-) ions held together by ionic bonds.


What is the iupac name of sodium iodide?

The IUPAC name for sodium iodide is sodium iodide.


What is the chemical name of Nal?

The chemical name of Nal is Naltrexone.


Formula of sodium plus Iodide?

The formula for sodium iodide is NaI. It is formed by the combination of sodium (Na) and iodide (I-) ions, with sodium donating an electron to iodine to form a stable compound.


What are the formulas for the ionic compounds named sodium iodide?

The formula for sodium iodide is NaI. It is composed of sodium ions (Na+) and iodide ions (I-), which combine in a 1:1 ratio to form a stable ionic compound through an electrostatic attraction.


Is sodium iodide iodide ionic or covalent?

Sodium iodide is an ionic compound. It is composed of sodium cations (Na+) and iodide anions (I-), which are held together by ionic bonds formed through the transfer of electrons from sodium to iodine.


Role of tris in TE in DNA elution?

Tris, commonly used as a buffering agent in Tris-EDTA (TE) buffer, helps to maintain the pH stability of the solution during DNA elution. Tris also provides a suitable ionic strength for DNA stability and helps to prevent degradation. It facilitates the solubilization of DNA during elution by providing a mild and stable environment.


What does sodium and iodine make?

Sodium and iodine combine to form sodium iodide (NaI). Sodium iodide is commonly used in medical treatments, diagnostic procedures, and as a nutritional supplement to address iodine deficiencies.


What is the percentage of sodium in sodium iodide?

Sodium iodide has a sodium cation and an iodide anion. The percentage of sodium in sodium iodide is 45.4%, as the molecular formula is NaI.


What is the name of the compound with the formula Nai?

NaI = Sodium iodide


Name the compound Nal?

The compound Nal is known as sodium iodide. It is commonly used in nuclear medicine for imaging procedures.


The formula for sodium iodide?

The chemical formula for sodium iodide is NaI.