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Because if the temperature is too high, it will result in both carbamylation of the protein due to the breakdown in urea and the aggregation of proteins. Hence 20 degrees is neither too high or too low for the IEF to run.

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11y ago
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4mo ago

The temperature of the IEF (Isoelectric focusing) step is typically set at 20 degrees Celsius to maintain a stable environment for the separation of proteins based on their isoelectric points. This temperature helps prevent protein denaturation while promoting optimal resolution and focusing of the proteins in the gel matrix.

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Q: Why is the temperature of the IEF step 20 degree?
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