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What is the name of VPO4?
Vanadium(III) phosphate is the name of the compound VPO4.
What are the standard chemicals used in lab for protein estimation?
There are several methods for protein estimation, these are the most common:Near UV Absorbance (280 nm). Quantitation of the amount of protein in a solution using a simple spectrophotometer. UV detection focuses on Tyr and Trp content, and to a very small extent on the amount of Phe and disulfide bonds.Far UV Absorbance (205 nm). Detects various side chains, including those of Trp, Phe, Tyr, His, Cys, Met, and Arg.These two methods uses: 0.1 M K2SO4 (pH 7.0), 5 mM KPO4 buffer (pH 7.0), Non -ionic detergent (e.g., 0.01% Brijn 35), and Guanidinium-HCl.The Lowry Method. This method is based on both the Biuret reaction, in wich the peptide bonds of proteins react with cooper under alkaline conditions to produce Cu+, which reacts with the Folin reagent, and the Folin-Ciocalteau reaction, where phosphomolybdotungstate is reduced to heteropolymolybdenum blue by the cooper-catalyzed oxidation of aromatic amino acids.This methods requires: Complex-forming reagent (composed by the Solution A: 2% (w/v) Na2CO3 in dH2O; Solution B: 1% (w/v) CuSO4 5H2O in dH2O; Solution C: 2% (w/v) sodium potassium tartrate in dH2O); 2 N NaOH; Folin Reagent (commercially available); and protein standards (bovine serum albumin fraction V is widely used).The Bicinchoninic Acid (BCA) Assay. This method is similar to Lowry method, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions. The Cu+ is then detected by reaction with BCA.This methods requires: Reagent A (0.1 g sodium bicinchoninate, 2.0 g Na2CO3 H2O, 0.16 g sodium tartrate dihydrate, 0.4 g NaOH, 0.95 g NaHCO3, made up to 100 mL. Adjust pH to 11.25 with NaHCO3 or NaOH, if necessary; Reagent B (0.4 g CuSo4 5H2O in 10 mL of water). The Standard Working Reagent (SWR) is made by mixing 100 vol of A + 2 vol of B.The Bradford Assay. This method relies on the binding of the dye Coomassie Blue G250 to protein.This assays requires a solution of 100 mg Coomassie Blue G250 in 50 mL of 95% ethanol, mixed with 100 mL of 85% phosphoric acid and completed to 1 L with distilled water.These are the most widely used assays for protein estimation.
