One mole of ammonium acetate is equal to 77.08g (this is the formula weight, FW, of ammonium acetate, which can be found on the side of the bottle). Another way of representing this is 77.08/mol (so, in one mole of ammonium acetate, there are 77.08grams of ammonium acetate).
We have to use the FW value to calculate molarity (moles of solute per L of solvent).
I am not sure what volume of the 50mM solution is desired, so I will assume that you need 1 L.
50mM is equal to 50milli-moles of solute/1 L of solvent, which is the same as 0.05moles/L. This is what the math looks like:
77.08g/mol ammonium acetate x 0.05mol/L = 3.854g/L
So, to make a 50mM solution of ammonium acetate in 1L of water, you will need to dissolve 3.854g of ammonium acetate into 1L of water.
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To make a 50mM ammonium acetate solution, dissolve the appropriate amount of ammonium acetate in a known volume of water to achieve a concentration of 50 millimoles per liter (mM). The molecular weight of ammonium acetate is 77.08 g/mol, so you would dissolve 3.854 g of ammonium acetate in 1 liter of water to make a 50mM solution.
To make 3 litres of 5mM Ammonium acetate solution, you would need to calculate the amount of Ammonium acetate needed based on its molecular weight. Once you have determined the mass needed, dissolve it in sufficient water to make the final volume of 3 litres. Keep in mind to use a balance to measure out accurate amounts of the compound for precise results.
To prepare a 0.38 M sodium acetate solution, you would need to dissolve the appropriate amount of sodium acetate trihydrate (CH₃COONa·3H₂O) in water. For example, to make 100 mL of a 0.38 M solution, you would dissolve 2.96 grams of sodium acetate trihydrate in sufficient water to make 100 mL. Ensure complete dissolution before use.
To make a 0.1N solution of ferrous ammonium sulfate, you first need to calculate the molecular weight of the compound. Then, you can dissolve the calculated amount of ferrous ammonium sulfate in the appropriate volume of water to prepare the desired concentration. Finally, make adjustments to the pH if needed.
To make a 6 N solution of ammonium hydroxide, you would mix the appropriate amount of concentrated ammonium hydroxide solution with water to achieve the desired concentration. The specific volumes of each component would depend on the concentration of the concentrated solution you are starting with. It is important to handle ammonium hydroxide with caution due to its caustic nature.
Sodium acetate buffer is a commonly used buffer solution in biochemical and molecular biology laboratories. It consists of a mixture of sodium acetate and acetic acid, and helps maintain a stable pH when added to solutions. It is effective in the pH range of around 4.7 to 5.7.