The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages.
Advantages:
Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method.
Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration.
Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods.
Disadvantages:
Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care.
Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods.
Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present.
In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
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