answersLogoWhite

0


Best Answer

Mass spec is done as a mass to charge ratio (m/z), so encouraging the compounds to have the same charge (by adding a bit of a low MW acid, for example ) is helpful in interpretation. You also have to do a calibration after setting the charge.

Positive ionization has been most common, and is used for peptides and proteins. Oligosaccharides, nucleotides and a few metals are typically done with negative ionization because their pKas are high and so it is more difficult to get positive ionization on them. Sometimes negative ion MS is used for mixtures because the abundance of the negative forms are lower and so the mixture is simpler to first analyze that way.

User Avatar

Wiki User

16y ago
This answer is:
User Avatar
More answers
User Avatar

AnswerBot

7mo ago

Negative ions are used in mass spectrometry because they tend to have higher sensitivity and selectivity for certain compounds, such as polar and acidic molecules. They also can produce unique fragmentation patterns that can help identify the compound of interest. Additionally, negative ion mode can help reduce background noise in the mass spectrum.

This answer is:
User Avatar

Add your answer:

Earn +20 pts
Q: Why use negative ions in mass spec?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Continue Learning about Chemistry

What symbols do we use to indicate that atoms have become ions?

We use superscripted charge symbols (+ or -) next to the element symbol to indicate that atoms have become ions. For example, Na+ represents a sodium ion with a positive charge, while Cl- represents a chloride ion with a negative charge.


How do you make a networked concept map of chemical bondings using the terms ionic covalent ions positive ions negative ions molecules polar and nonpolar?

To create a networked concept map of chemical bondings using these terms, you can start by categorizing them into groups like bond types (ionic, covalent), species involved (ions, molecules), and polarity (polar, nonpolar). Then, connect them based on relationships such as how ionic bonds form between positive and negative ions, covalent bonds between atoms within molecules, and how polarity affects molecular interactions. You can use arrows and labels to show these connections clearly in your concept map.


Does the pH scale measure the concentration of hydroxide ions?

No, the pH scale measures the concentration of hydrogen ions in a solution. The concentration of hydroxide ions is related to the pH through the relationship pH + pOH = 14.


How many moles of ions are present in aqueous solutions prepared by dissolving 10.00g of the f scandium III bromide in water to make a 1.25 L solution?

To find the number of moles of ions, first calculate the molar mass of scandium(III) bromide (ScBr3). The molar mass is approximately 280.79 g/mol. Next, use the given mass of 10.00g to find the number of moles of ScBr3. Then, recognize that every formula unit of ScBr3 dissociates into 4 ions in solution (1 Sc3+ ion and 3 Br- ions), so multiply the number of moles of ScBr3 by 4 to find the total number of moles of ions.


How do go from moles to ions?

To go from moles to ions, you need to know the ionic formula of the compound. Calculate the moles of the compound using its molar mass, and then use the ratio of ions in the formula to determine the number of ions present. For example, in NaCl (sodium chloride), 1 mole of NaCl yields 1 mole of Na+ ions and 1 mole of Cl- ions.

Related questions

What is the use of TOF in massspectrometer?

The ToF is the mass separator, distinguishing ions by their mass in the same way as the magnetic sector does in a magnetic sector mass spec.


What does electrolysis use to separate molecules in a chemical change?

The electrolysis ionize the particles/ molecules present in the particles and charged the positive and negative ions. Positive ions collected on negative electrode and Negative ions collected on positive electrodes.


How do you make negative ion?

Negative ions are naturally produced in the environment through processes such as sunlight, water movement, and thunderstorms. You can also generate negative ions indoors using devices like air ionizers, which use electricity to create negative ions by releasing them into the air, helping to improve air quality by removing pollutants.


What W can be used to dissolve salt?

Water can be use to dissolve salt. The positive part of water molecules attracts the negative chloride ions and the negative part of water molecules attracts the positive sodium ions.


Why Nokia BTS use minus 48volt?

TELECOM COMPANIES USE MINUS 48 RATHER THAN POSITIVE TO AVOID THE CORROSION IN THE EQUIPMENT AND WIRE AS WELL AS. THERE ARE NEGATIVE IONS PRESENT IN AIR AND DIRT ARE RESPONSIBLE FOR CORROSION THERE ARE TWO MAIN IONS OXYGEN AND CARBONATE THESE ARE NEGATIVE IONS SO THAT IF WE EQUIP OUR EQUIPMENT POSITIVE THEN THIS WILL ATTRACT THESE IONS AND WILL BE MORE CORROSION AND IF THIS WILL BE NEGATIVE THEN THIS WILL REPEL AND WILL REDUCE THE CORROSION AND EQUIPMENT WILL BE LONG LASTING. BR// Paritosh tiwari lucknow ( INDIA )


Which reagents do you use to test for negative ions?

There is not a special test for negative ions IN GENERAL. However there are many possibillities for negative ions of each kind in particular: Example: test on Cl- : add silver nitrate: AgCl precipitate test on S2-: add drop of dilute acid: smell of rotten eggs (H2S) test on SO42-: add BaCl2 solution: BaSO4 precipitate


What is more correct negative correlation or inverse correlation?

Those are both perfectly valid terms, which you would use according to context. You might say, for example, that obesity has a negative correlation to longevity. And in an aqueous solution there is an inverse correlation between hydrogen ions and hydroxide ions.


What symbols do we use to indicate that atoms have become ions?

We use superscripted charge symbols (+ or -) next to the element symbol to indicate that atoms have become ions. For example, Na+ represents a sodium ion with a positive charge, while Cl- represents a chloride ion with a negative charge.


Why do you use the term formula mass instead of molecular mass for ionic compound?

We use the term formula mass for ionic compounds because they do not exist as discrete molecules like covalent compounds. Instead, ionic compounds exist as a three-dimensional array of ions arranged in a crystal lattice. Therefore, the term formula mass better reflects the overall mass of the compound, considering all the atoms and ions present in its formula unit.


How do you count the number of ions in a equation?

To count the number of ions in an equation, first identify the ions present in each compound. Then, determine the number of each type of ion by looking at the subscripts in the chemical formula. Finally, multiply the number of ions by the coefficient (if present) to get the total number of ions in the equation.


A volume of 10 ml of a .00600 M solution of Cl- ions are reacted with 0.500 m solution of AgNO3 what is the maximum mass of AgCL that precipitates?

To determine the maximum mass of AgCl precipitated, we need to calculate the limiting reactant. First, calculate the moles of Cl- ions in the 10 ml solution. Then, use the mole ratio from the balanced equation between Cl- ions and Ag+ ions to find the moles of Ag+ ions available for reaction. Finally, convert the moles of AgCl formed to grams using the molar mass of AgCl to find the maximum mass precipitated.


How do you make a networked concept map of chemical bondings using the terms ionic covalent ions positive ions negative ions molecules polar and nonpolar?

To create a networked concept map of chemical bondings using these terms, you can start by categorizing them into groups like bond types (ionic, covalent), species involved (ions, molecules), and polarity (polar, nonpolar). Then, connect them based on relationships such as how ionic bonds form between positive and negative ions, covalent bonds between atoms within molecules, and how polarity affects molecular interactions. You can use arrows and labels to show these connections clearly in your concept map.