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Hydrochloric acid (HCl) is used in dilution for hemoglobin count because it helps to release hemoglobin from red blood cells, making it easier to measure accurately in laboratory tests. By breaking down the red blood cells, HCl facilitates the release of hemoglobin for a more precise quantification.
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Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
How do you get 0.1M from 1M HCL?
To make a 0.1M solution from a 1M HCL solution, you would dilute the 1M HCL with 10 parts of water (or whatever solvent you are using). For example, mix 1 mL of 1M HCL with 9 mL of water to obtain a 0.1M HCL solution.
How do you turn 1N of HCL into 0.001N of HCL?
You can dilute the 1N HCl solution by adding 999 parts of water to 1 part of the 1N HCl solution. For example, take 1 mL of 1N HCl and add it to 999 mL of water to create a 0.001N HCl solution.
How do you make 5mM HCl?
To make a 5mM solution of HCl, you would need to dilute concentrated HCl (usually in the range of 10-12M) with water. The exact dilution volume will depend on the concentration of the concentrated HCl solution you have. It's crucial to wear appropriate personal protective equipment and handle concentrated HCl with care due to its corrosive nature.
Why do you wait after adding the blood to hcl for ten minutes in sahli's method of hb?
Waiting for 10 minutes after adding blood to HCl in Sahli's method allows for complete lysis of red blood cells and release of hemoglobin. This step ensures that hemoglobin is in its free form and stable for accurate measurement.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Why hcl is used in Hb estimation?
HCl (hydrochloric acid) is used in the estimation of hemoglobin (Hb) because it helps to release hemoglobin from red blood cells, making it more accessible for measurement. It also helps to remove other interfering substances, ensuring a more accurate estimation of hemoglobin levels.
What is Acid hematin Method?
it is formed when hcl reacts with hemoglobin ................it is the end product
How do you get 0.1M from 1M HCL?
To make a 0.1M solution from a 1M HCL solution, you would dilute the 1M HCL with 10 parts of water (or whatever solvent you are using). For example, mix 1 mL of 1M HCL with 9 mL of water to obtain a 0.1M HCL solution.
How do you turn 1N of HCL into 0.001N of HCL?
You can dilute the 1N HCl solution by adding 999 parts of water to 1 part of the 1N HCl solution. For example, take 1 mL of 1N HCl and add it to 999 mL of water to create a 0.001N HCl solution.
How do you make 5mM HCl?
To make a 5mM solution of HCl, you would need to dilute concentrated HCl (usually in the range of 10-12M) with water. The exact dilution volume will depend on the concentration of the concentrated HCl solution you have. It's crucial to wear appropriate personal protective equipment and handle concentrated HCl with care due to its corrosive nature.
Why do you wait after adding the blood to hcl for ten minutes in sahli's method of hb?
Waiting for 10 minutes after adding blood to HCl in Sahli's method allows for complete lysis of red blood cells and release of hemoglobin. This step ensures that hemoglobin is in its free form and stable for accurate measurement.
How will you prepare one liter of 0.1 N HCL solution from 12 N HCL?
To prepare 1 liter of 0.1N HCl solution from 12N HCl, you would need to dilute the 12N HCl by a factor of 120. To do this, you would add approximately 83.33 mL of 12N HCl to a container and then dilute it with water to reach a final volume of 1 liter. Make sure to mix the solution thoroughly after dilution.
How many mL of conc. HCl equals 1 Liter 0.05 molar Hydrochloric acid?
To make 1 liter of 0.05 M HCl solution, you would need to dilute concentrated HCl. The concentration of the concentrated HCl would depend on its specific concentration, but you would typically need around 100 mL of concentrated HCl, assuming it is a standard 12 M concentration, to make the desired dilution.
How do you make 1M HCl from 12M HCl?
To make 1M HCl from 12M HCl, you can dilute the 12M HCl by adding water. The dilution process involves adding a specific amount of 12M HCl to a larger volume of water to achieve the desired concentration of 1M HCl. Calculate the volume of 12M HCl and water needed using the dilution formula C1V1 = C2V2, where C1 and V1 are the initial concentration and volume of the 12M HCl, and C2 and V2 are the desired concentration and volume of the final 1M HCl solution. Mix the solution thoroughly to ensure proper mixing.
Is Ciproflocaxin hcl used to treat BV?
Ciproflocaxin hcl isn't used to treat BV.
What are the concentrations of HCl?
The concentration of HCl (hydrochloric acid) can vary depending on its intended use. Common concentrations include 0.1 M (molar), 1 M, and concentrated HCl which is around 12 M. It is important to always check the label or specification sheet for the exact concentration of the HCl solution.
