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The pigment spot on chromatography paper should not be submerged in solvent because it will result in spreading and mixing of the pigments, making it difficult to distinguish them. Instead, the solvent should be allowed to slowly travel up the paper by capillary action, separating the pigments based on their solubility and mobility.
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What are the two types of paper chromatogaphy?
The two types of paper chromatography are ascending chromatography, where the solvent moves up the paper, and descending chromatography, where the solvent moves down the paper.
What would happen if you submerge the chromatography paper above the pencil line?
Submerging the chromatography paper above the pencil line would cause the solvent to rise above the line, potentially leading to the ink or pigment above the line to dissolve and be carried upwards with the solvent. This can result in the separation of different components within the ink or pigment.
Why solvent is used in Paper chromatography?
Solvent is used in paper chromatography to carry the sample mixture along the paper and separate its components based on their affinity for the solvent and the paper. As the solvent moves through the paper, it dissolves the components of the sample and allows them to separate based on their solubility and interactions with the paper.
What do the Rf values indicate about the relative solubility of the pigment in the the solvent?
The Rf value indicates the ratio of the distance a pigment travels compared to the distance the solvent travels on a chromatography plate. The higher the Rf value, the more soluble the pigment is in the solvent used for the chromatography.
How is the retention factor measured in paper chromotography is related to pigmnet color in leaves?
The retention factor (Rf value) in paper chromatography is calculated as the distance the pigment traveled divided by the distance the solvent front traveled. The Rf value is unique for each pigment and helps identify and compare different pigments based on their mobility during chromatography. Pigments with higher Rf values move further up the paper, showing greater solubility, while pigments with lower Rf values stay closer to the solvent front.
What are the two types of paper chromatogaphy?
The two types of paper chromatography are ascending chromatography, where the solvent moves up the paper, and descending chromatography, where the solvent moves down the paper.
Which determines the speed with which a pigment moves up the chromatography paper?
Chromatography is a technique that separates molecules from each other on the basis of their solubility in particular solvents. As a nonpolar solvent moves up the chromatography paper, the pigment moves along iwth it. The more non-polar a pigment, the more soluble it is in a nonpolar solvent, and the faster and father it proceeds up the chromatography. Pg 94, laboratory 8.1, Inquiry into Life, Sylvia S. Mader, laboratory manual, 12th edition
What determines the distance a pigment moves up the paper?
The distance a pigment travels up the paper in paper chromatography is determined by its solubility in the solvent being used, its molecular size, and its attraction to the paper. Pigments that are more soluble and smaller in size tend to move further up the paper, while those with stronger interactions with the paper tend to stay closer to the origin.
Will water extract pigment in a chromatography?
Yes, water can extract pigment during chromatography. This technique uses a solvent to separate and analyze mixtures, and water is often used as the solvent for certain types of pigments. Water's polarity can help separate different pigments based on their solubility and interactions with the paper or gel used in chromatography.
What would happen if you submerge the chromatography paper above the pencil line?
Submerging the chromatography paper above the pencil line would cause the solvent to rise above the line, potentially leading to the ink or pigment above the line to dissolve and be carried upwards with the solvent. This can result in the separation of different components within the ink or pigment.
Why solvent is used in Paper chromatography?
Solvent is used in paper chromatography to carry the sample mixture along the paper and separate its components based on their affinity for the solvent and the paper. As the solvent moves through the paper, it dissolves the components of the sample and allows them to separate based on their solubility and interactions with the paper.
Why does carotene travel farthest in chromatography of leaf pigments?
Carotene travels the farthest in chromatography of leaf pigments because it is the least soluble in the chromatography solvent. This means it interacts less with the solvent and more with the chromatography paper, allowing it to move further up the paper before the solvent front stops it.
What is descending paper chromatography?
Descending paper chromatography is a type of chromatography technique where a sample solution is applied near the top of a strip of chromatography paper and then allowed to flow down the paper in a solvent. As the solvent moves down the paper, it carries the components of the sample at different rates based on their affinity for the paper and the solvent. This separation allows for the identification and quantification of the components in the sample.
What two factors determine the rate at which the pigments travel up the chromatography paper?
Rf value. polarity of solvent
Which pigment in chlorophyll moves the fastest?
Chlorophyll b typically moves faster than chlorophyll a during paper chromatography due to its higher polarity and increased ability to interact with the paper and solvent.
What do the Rf values indicate about the relative solubility of the pigment in the the solvent?
The Rf value indicates the ratio of the distance a pigment travels compared to the distance the solvent travels on a chromatography plate. The higher the Rf value, the more soluble the pigment is in the solvent used for the chromatography.
How is the retention factor measured in paper chromotography is related to pigmnet color in leaves?
The retention factor (Rf value) in paper chromatography is calculated as the distance the pigment traveled divided by the distance the solvent front traveled. The Rf value is unique for each pigment and helps identify and compare different pigments based on their mobility during chromatography. Pigments with higher Rf values move further up the paper, showing greater solubility, while pigments with lower Rf values stay closer to the solvent front.
