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A requirement of the gel is that it is neutral and that included the absence of charged impurities. If the gel contains any charged groups then an effect known as electroendosmosis will take place.
As these charged groups are immobilised onto the gel they cause a solvent flow towards one of the electrodes, usually the cathode (negative) and thus in opposition to the sample flow.
This is obviously undesirable as this will slow down and may distort the migration of the samples reducing resolution which can compromise quantitative accuracy and precision.
Operation at extremes of pH to minimize these problems is useful in special cases but is not a general strategy for protein separations
Alkaline conditions are used in electrophoresis to denature DNA, which helps in separating the two strands. This allows for more precise separation and analysis of DNA fragments based on size. Additionally, alkaline conditions also help in preventing DNA molecules from reannealing during electrophoresis.
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What enzyme works best in alkaline?
Alkaline phosphatase is an enzyme that works best in alkaline conditions. It is commonly used in molecular biology and biochemistry research due to its optimal activity in alkaline pH levels.
What colour is methyl orange in alkaline?
Methyl orange is yellow in alkaline conditions.
What colour is methyl yellow when it reacts with an alkali?
Methyl yellow is yellow in acidic conditions, but changes to red in alkaline conditions.
How does the liver help to provide alkaline conditions?
The liver helps to provide alkaline conditions in the body by producing and secreting bile, which is a basic or alkaline solution. Bile helps to neutralize acids from the stomach and aids in digestion in the small intestine, contributing to an overall alkaline environment in the digestive system.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What enzyme works best in alkaline?
Alkaline phosphatase is an enzyme that works best in alkaline conditions. It is commonly used in molecular biology and biochemistry research due to its optimal activity in alkaline pH levels.
What would altered conditions in electrophoresis do?
who was the first astronomer to see mountains on the moom
What colour is methyl orange in alkaline?
Methyl orange is yellow in alkaline conditions.
What was used before gel electrophoresis?
Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
What are the conditions of the small intestine like?
Alkaline
How do you test which enzymeproteaseamylaseand catalase will work better in alkaline conditions?
Put them all into separate alkaline conditions and then introduce their respective substrate and observe the reaction.
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
Is electrophoresis part of biotechnology?
Yes, electrophoresis is a common technique used in biotechnology. It is used to separate and analyze macromolecules such as DNA, RNA, and proteins based on their size and charge. Electrophoresis plays a crucial role in various applications, including genetic analysis, protein characterization, and forensic studies.
The role of ammonia in Tollen?
Ammonia is used as a ligand in order to dissolve the insoluble Silver ions under alkaline conditions.
What colour is methyl yellow when it reacts with an alkali?
Methyl yellow is yellow in acidic conditions, but changes to red in alkaline conditions.
What is used to sort DNA into lengths?
Gel Electrophoresis
