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Garlic (Allium sativum L.) and garlic extracts have therapeutical properties that stem from their sulfur-containing compounds, mainly allicin. The main objective of this work was to compare conventional and "premium" garlic extracts in terms of yield and quality, with the latter being obtained using supercritical carbon dioxide (SC-CO2) as the solvent. Yield ranged between 0.65 and 1.0% and increased with extraction pressure (150-400 bar) at a constant temperature of 50°C. Extraction temperature (35-60°C), on the other hand, had little effect at a constant pressure of 300 bar. Based on yield and quality considerations, the best extraction conditions using SC-CO2 were 35-50°C and 300-400 bar. A yield of 5.5% was obtained by conventional extraction using ethanol as the solvent, but ethanol appeared to be less selective for valuable components than SC-CO2. The use of fresh garlic resulted in extracts that more closely resembled commercial products, possibly because of thermal and oxidative degradation of valuable microconstituents during drying.
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What is the role of 70 ethanol in DNA extraction?
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
Why 75 percent ethanol is used in rna extraction?
75% ethanol is commonly used in RNA extraction because it helps to wash the RNA pellet by removing salts and other contaminants, while also helping to maintain the integrity and stability of RNA molecules. The lower ethanol concentration reduces the risk of RNA degradation and allows for efficient RNA recovery during the extraction process.
Which cell parts were dissolved in the ethanol?
Chlorophyll and other pigments in the chloroplasts were dissolved in the ethanol during the extraction process, leading to the green color of the solution.
What alcohol and acid form ethyl acetate?
Ethanol and acetic acid react to form ethyl acetate, with water as a byproduct. This reaction is commonly used in laboratories and industrial settings to produce ethyl acetate for various applications such as solvent extraction and flavoring.
Why use dichloromethane instead of ethanol to extract trimyristin?
Dichloromethane is often preferred over ethanol for extracting trimyristin because it is a non-polar solvent that can effectively dissolve the target compound. Additionally, dichloromethane has a lower boiling point than ethanol, making it easier to separate from the extracted trimyristin. Lastly, dichloromethane is less polar than ethanol, which can lead to a more efficient extraction process.
Can you use ethanol in extraction with water?
Yes, ethanol can be used in extraction with water. This process is known as a liquid-liquid extraction or partitioning. Ethanol can help solubilize certain compounds that are not soluble in water, allowing for the extraction of a wider range of compounds from the sample.
What is the role of 70 ethanol in DNA extraction?
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
Why is 70 percent ethanol used in rna extraction?
Seventy percent ethanol is commonly used in RNA extraction to wash and remove salts and contaminants from the RNA sample. It helps to purify the RNA by precipitating it out of the solution while leaving behind impurities. Additionally, the 70% ethanol concentration helps minimize RNA degradation during the extraction process.
What is preferred in DNA extraction ethanol or isopropanol and why?
Isopropanol is more preferred than ethanol in DNA extraction, as isopropanol facilitates precipitation more better, as it possess very less i.e., 0.6 to 0.7 volumes of alcohol.
Why 75 percent ethanol is used in rna extraction?
75% ethanol is commonly used in RNA extraction because it helps to wash the RNA pellet by removing salts and other contaminants, while also helping to maintain the integrity and stability of RNA molecules. The lower ethanol concentration reduces the risk of RNA degradation and allows for efficient RNA recovery during the extraction process.
Which cell parts were dissolved in the ethanol?
Chlorophyll and other pigments in the chloroplasts were dissolved in the ethanol during the extraction process, leading to the green color of the solution.
Where are there instructions on how exactly to extract LSA from morning glory seeds?
A VERY brief overview of the extraction process: Grind Ipomoea seeds. Soak grounds in nonpolar solution. Filter out (then throw out) bulk of liquid, evaporate remainder. Soak in polar solution (ethanol), throw out grounds, evaporate ethanol, and you have your product. Found this on a google search. There is a million ways listed, all involve alcohol, (ethanol).
What does sodium citrate do in DNA extraction?
Sodium citrate is used in DNA extraction to help neutralize the charge on DNA molecules, making them more insoluble in alcohol. This helps to precipitate the DNA out of solution, allowing for easier isolation and purification of the DNA.
Would ethanol be a good solvent for extracting caffeine from water?
Yes, ethanol can be a good solvent for extracting caffeine from water. Ethanol is a polar solvent that can effectively dissolve caffeine, making it suitable for extraction purposes. It is also commonly used in many extraction processes due to its efficient solubility properties.
What is the purpose of soap in strawberry extraction?
The purpose is to help the mixture of salt water and ethanol so the can find the DNA of strawberry bananna etc. Extrsctions
What are the uses of ethanol in microbiology lab?
Ethanol is commonly used in microbiology labs as a disinfectant to sterilize surfaces, equipment, and lab benches. It is also used for flame sterilization of inoculating loops and needles. Additionally, ethanol is used in DNA and RNA extraction protocols to precipitate nucleic acids.
What is a good alternative to hexane?
Super critical carbon dioxide is my favorite but one can use ethanol propane and enzymatic aqueous extractions as well as mechanical press extraction.
