Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl).
This method requires the following reagents
1. Orcinol reagent : 6% orcinol in 95% ethanol.
2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.))
Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test.
This method requires the following reagents
one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization.
Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution.
This method requires the following reagents
Hydrochloric acid, 1Normal
Schiff's reagent (made from pararosanilin treated with sulphurous acid)
Light green, 1% aqueous (can be replaced with Fast green FCF)
For nucleic acids, commonly used testing indicators include ethidium bromide and SYBR Green, which fluoresce when bound to DNA or RNA, allowing visualization under ultraviolet light. These indicators are used in techniques like agarose gel electrophoresis and quantitative PCR to detect and quantify nucleic acids.
Nucleic acids and carbohydrates are types of biomolecules.
Yes, nucleic acids are polymers made up of repeating units called nucleotides. RNA and DNA are examples of nucleic acids that contain long chains of nucleotide monomers.
Nucleic acids are biological molecules that store and transmit genetic information within a cell. They are made up of building blocks called nucleotides, which consist of a sugar, a phosphate group, and a nitrogenous base. DNA and RNA are examples of nucleic acids.
Yes, bacon contains nucleic acids because it is a meat product derived from an animal, which naturally contains nucleic acids in its cells. Nucleic acids are essential components of all living organisms and are found in both plant and animal tissues.
No, fingernails are not nucleic acids. Nucleic acids are biological macromolecules like DNA and RNA that store and transmit genetic information, while fingernails are made of a tough protein called keratin.
Nucleic acids
Nucleic acids are polymers of nucleotides.
They are the nucleotides . They make up nucleic acids
No. Nucleic acids are the building blocks of protein. There are various types of nucleic acids that form proteins.
No. Nucleic acids encode proteins.
Nucleic acids. Dna = Deoxyribonucleic Acid Rna = Ribonucleic Acid
Nucleic acids make proteins.
The four major macromolecules are proteins, carbohydrates, lipids, and nucleic acids.
Nucleic acids.
No, nucleic acids are molecules that store and transmit genetic information, such as DNA and RNA, while amino acids are the building blocks of proteins. Proteins are made up of chains of amino acids, not nucleic acids.
Nucleic Acids Research was created in 1974.
RNA and DNA are nucleic acids because they are composed of chains of nucleotides, which make up nucleic acids.