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Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl).
This method requires the following reagents
1. Orcinol reagent : 6% orcinol in 95% ethanol.
2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.))
Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test.
This method requires the following reagents
one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization.
Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution.
This method requires the following reagents
Hydrochloric acid, 1Normal
Schiff's reagent (made from pararosanilin treated with sulphurous acid)
Light green, 1% aqueous (can be replaced with Fast green FCF)
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JordanFor nucleic acids, commonly used testing indicators include ethidium bromide and SYBR Green, which fluoresce when bound to DNA or RNA, allowing visualization under ultraviolet light. These indicators are used in techniques like agarose gel electrophoresis and quantitative PCR to detect and quantify nucleic acids.
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Are nucleic acids organic or inorganic compounds?
Nucleic acids are organic compounds.
Nucleic acids and carbohydrates are a type of what?
Nucleic acids and carbohydrates are types of biomolecules.
Do nucleic acids have polymers?
Yes, nucleic acids are polymers made up of repeating units called nucleotides. RNA and DNA are examples of nucleic acids that contain long chains of nucleotide monomers.
What statement best describes nucleic acids?
Nucleic acids are biological molecules that store and transmit genetic information within a cell. They are made up of building blocks called nucleotides, which consist of a sugar, a phosphate group, and a nitrogenous base. DNA and RNA are examples of nucleic acids.
Does bacon have nucleic acids?
Yes, bacon contains nucleic acids because it is a meat product derived from an animal, which naturally contains nucleic acids in its cells. Nucleic acids are essential components of all living organisms and are found in both plant and animal tissues.
