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The Beer-Lambert law

Absorbance = (extinction coefficent)(pathlength of light)(concentration)

allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time.

the pathlength of light being the width of the cuvette

and the extinctin coefficent being specific to the product molecule.

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βˆ™ 7mo ago

Beer-Lambert's law is used in UV-Visible spectrophotometry to relate the concentration of a sample to the absorbance of light passing through it. This law states that the absorbance is directly proportional to the concentration of the absorbing species and the pathlength of the sample. It is a fundamental principle in spectroscopy for quantifying the concentration of a substance in a solution.

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Q: What is the relation of Beers Lambert's law in UV Visible spectrometer?
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