0
What else can I help you with?
Who invented rt-pcr?
Kary Mullis is credited with inventing the polymerase chain reaction (PCR) technique in the 1980s, but reverse transcription PCR (RT-PCR) was a further development by researchers like A. W. Higuchi that allowed for the amplification of RNA sequences.
Describe the locations reactants and products of transcription and translation?
Transcription is located in the nucleus, and translation is located in the cytoplasm. The product of transcription is RNA, and the product of translation is protein. The reactants for transcription is DNA, and the reactants for translation is mRNA and tRNA.
How did scientists convent information when using reverse transcriptase in insulin research?
Scientists used reverse transcriptase in insulin research by first isolating messenger RNA (mRNA) from cells that produce insulin. The reverse transcriptase enzyme then converted this mRNA into complementary DNA (cDNA), which could be used for further analysis and cloning. This allowed researchers to study the genetic basis of insulin production and potentially manipulate it for various applications.
What substance can be used to stop enzyme reaction?
Denaturing agents such as heat, extreme pH levels, or organic solvents can be used to stop enzyme reactions by altering the enzyme's structure and activity. Additionally, specific enzyme inhibitors can be used to block the active site or prevent substrate binding, effectively stopping the enzymatic reaction.
Is p-nitrophenol an enzyme?
No, p-nitrophenol is not an enzyme. It is a chemical compound that is often used in biochemical research as a substrate for enzyme assays.
Which Enzyme is capable of reverse transcription?
Rna reverse transcriptase.
Which process produces RNA to dna?
The process that produces DNA from RNA is called reverse transcription. This process is carried out by an enzyme called reverse transcriptase, which converts RNA into DNA, allowing the genetic information from the RNA to be transcribed into DNA. Reverse transcription is commonly used by retroviruses, such as HIV, as part of their replication cycle.
Which enables DNA to be synthesize from RNA?
The reverse transcriptase enzyme is responsible for synthesis of DNA from an RNA template by the process of reverse transcription.
Reverse transcription occurs when?
Reverse transcription occurs when RNA is converted into DNA by the enzyme reverse transcriptase. This process is commonly observed in retroviruses like HIV, where the viral RNA is reverse transcribed into DNA upon infection of a host cell. The resulting DNA can then integrate into the host cell's genome and be used as a template for viral replication.
Reverse transcription 5' to 3'?
Reverse transcription is the process of synthesizing a DNA molecule from an RNA template. In this process, a reverse transcriptase enzyme catalyzes the formation of DNA nucleotides in the 5' to 3' direction, complementary to the RNA template. This results in the creation of a DNA molecule that is a copy of the original RNA molecule.
What is the enzyme that catalyzes transcription?
The enzyme that catalyzes transcription is called RNA polymerase.
What enzyme is needed to turn a RNA virus into a DNA virus?
The enzyme needed to convert RNA into DNA in a process called reverse transcription is called reverse transcriptase. This enzyme is unique to retroviruses, such as HIV, which use it to convert their RNA genome into DNA once they infect a host cell.
What enzyme converts RNA to DNA?
The enzyme that converts RNA to DNA is called reverse transcriptase. This enzyme is commonly found in retroviruses and is utilized in the process of reverse transcription, where RNA is used as a template to synthesize a complementary DNA strand.
RNA can manufacture DNA via the action of what?
RNA can manufacture DNA via the action of reverse transcriptase, an enzyme found in retroviruses. Reverse transcriptase helps transcribe RNA into DNA by synthesizing a complementary strand of DNA based on the RNA template.
What enzyme catalyzes transcription in cells?
The enzyme that catalyzes transcription in cells is called RNA polymerase.
What is the process making RNA to DNA?
The process of converting RNA to DNA is called reverse transcription. This process is carried out by the enzyme reverse transcriptase, which uses an RNA template to synthesize a complementary DNA strand. Reverse transcription is a key step in the replication of retroviruses like HIV and in the production of complementary DNA (cDNA) from RNA for use in research applications.
Which enzyme binds to DNA during transcription?
RNA polymerase is the enzyme that binds to DNA during transcription.
