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It is a test for unsaturated steroids (as cholesterol) and triteness based on the formation of a series of colors (as pink to blue to green) with acetic an-hydride in the presence of concentrated sulfuric acid called also Liebermann-Burchard test

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15y ago
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6mo ago

The Lieberman-Burchard test is a colorimetric test used to detect the presence of cholesterol. The test involves the addition of sulfuric acid and acetic anhydride to a sample containing cholesterol, resulting in a color change from red to green or blue if cholesterol is present. The test is sensitive and specific for cholesterol but cannot differentiate between different sterols.

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11y ago

Lieberman-Burchard is a reagent used in a colourimetric test to detect cholesterol.

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Q: Principles of lieberman-burchard test
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