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Equation for the ethyl acetate test?
The ethyl acetate test is a qualitative test used to detect the presence of acetoacetic acid in urine. The equation involves the reaction of acetoacetic acid in urine with ethyl acetate in the presence of a mineral acid like hydrochloric acid. The equation can be represented as: CH3COCH2COOH (acetoacetic acid) + CH3COOCH2CH3 (ethyl acetate) + HCl → no visible change A positive result is indicated by the production of a pink or red color in the upper ethyl acetate layer due to the formation of sodium nitroprusside complex with acetoacetic acid.
What is acetyl acetic acid?
Acetyl acetic acid is also known as acetoacetic acid. It is a beta-keto acid and is an important intermediate in the biosynthesis of fatty acids and cholesterol. It is also a key component in the ketone body metabolism pathway.
Why Rothera's test not used to detect beta hydroxy butryic acid?
Rothera's test is not specific for beta-hydroxybutyric acid because it primarily detects acetone and acetoacetic acid. Beta-hydroxybutyric acid does not yield a positive reaction in Rothera's test due to its chemical structure differing from that of acetone and acetoacetic acid. Therefore, alternative tests such as enzymatic assays or ketone dipstick tests are preferred for detecting beta-hydroxybutyric acid.
How does nitroprusside reaction works?
Nitroprusside reacts with nitric oxide in the body to release cyanide ions, which can be toxic. To minimize toxicity, nitroprusside is typically administered in a controlled setting with continuous monitoring of cyanide levels. It is used as a vasodilator to lower blood pressure in emergencies.
Is chloridric acid same as hydrochloric acid?
No, chloridric acid is not the same as hydrochloric acid. Chloridric acid is a term that is sometimes used interchangeably with hydrochloric acid, but the correct name for the compound is hydrochloric acid. They both refer to the same compound, which is a strong, corrosive acid with the formula HCl.
What is an acetoacetate?
An acetoacetate is a salt or ester of acetoacetic acid.
What is an acetoacetyl?
An acetoacetyl is a radical derived from acetoacetic acid.
Equation for the ethyl acetate test?
The ethyl acetate test is a qualitative test used to detect the presence of acetoacetic acid in urine. The equation involves the reaction of acetoacetic acid in urine with ethyl acetate in the presence of a mineral acid like hydrochloric acid. The equation can be represented as: CH3COCH2COOH (acetoacetic acid) + CH3COOCH2CH3 (ethyl acetate) + HCl → no visible change A positive result is indicated by the production of a pink or red color in the upper ethyl acetate layer due to the formation of sodium nitroprusside complex with acetoacetic acid.
What are the types of ketone bodies present in urine?
The types of ketone bodies present in urine are acetoacetate, beta-hydroxybutyrate, and acetone. These compounds are produced as by-products of fatty acid metabolism when the body is in a state of ketosis, such as during fasting or low-carbohydrate diets. Detection of ketone bodies in urine can indicate metabolic imbalance or disorders like diabetes or ketoacidosis.
Is Acetoacetate a ketone body?
Yes, acetoacetate is a ketone body produced as a byproduct of fatty acid metabolism. It is one of the three main ketone bodies, along with beta-hydroxybutyrate and acetone, that are used as alternative sources of energy when glucose availability is low, such as during fasting or ketogenic diets.
What is the difference between malonic ester synthesis and acetoacetic ester synthesis?
Malonic ester synthesis involves the reaction of diethyl malonate with a base and alkyl halide to form a substituted carboxylic acid. Acetoacetic ester synthesis involves the reaction of ethyl acetoacetate with a base and alkyl halide to form a substituted ketone. Both methods are used to synthesize β-ketoesters, but the starting materials and products differ.
What is acetyl acetic acid?
Acetyl acetic acid is also known as acetoacetic acid. It is a beta-keto acid and is an important intermediate in the biosynthesis of fatty acids and cholesterol. It is also a key component in the ketone body metabolism pathway.
Chemical equation for manufacturing of dane salt?
Parahydroxy phenyl glycine, Potassium hydroxide & Methyl acetoacetate reacts to form a salt named as Dane Salt. Its a simple reaction. Potassium hydroxide forms potassium salts at carboxylic acid chain of glycine & Methyl acetoacetate forms salt at the amino chain of glycine.
Why Rothera's test not used to detect beta hydroxy butryic acid?
Rothera's test is not specific for beta-hydroxybutyric acid because it primarily detects acetone and acetoacetic acid. Beta-hydroxybutyric acid does not yield a positive reaction in Rothera's test due to its chemical structure differing from that of acetone and acetoacetic acid. Therefore, alternative tests such as enzymatic assays or ketone dipstick tests are preferred for detecting beta-hydroxybutyric acid.
How does nitroprusside reaction works?
Nitroprusside reacts with nitric oxide in the body to release cyanide ions, which can be toxic. To minimize toxicity, nitroprusside is typically administered in a controlled setting with continuous monitoring of cyanide levels. It is used as a vasodilator to lower blood pressure in emergencies.
How do you synthesise 11-diphenyl-1-hydroxy-3-butanone from ethyl acetoacetate?
To synthesize 1-hydroxy-3-butanone from ethyl acetoacetate, first convert ethyl acetoacetate to ethyl acetoacetate phenylhydrazone using phenylhydrazine. Then, cyclize the phenylhydrazone to form the desired 1-hydroxy-3-butanone product. Hydrolyze the phenylhydrazone under acidic conditions to obtain the final product, 11-diphenyl-1-hydroxy-3-butanone.
Why acetoacetic ester gives ferric chloride test?
Acetoacetic ester gives a positive ferric chloride test due to the presence of the beta-keto group in the molecule. The keto group reacts with ferric chloride to form a colored complex, producing a characteristic color change that indicates the presence of the keto group in the compound.
