Gram negative
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Without mordant, E. coli would appear pink or red after Gram staining due to retaining the safranin counterstain, indicating that it is a Gram-negative bacterium. The absence of the mordant would prevent the crystal violet stain from binding strongly to the peptidoglycan layer in the cell wall, leading to this coloration.
No, sanding off the galvanized coating from steel does not provide a suitable surface for etching in mordant. The coating needs to be removed using a chemical stripping process for successful etching with mordant on steel. Look for specialized products designed for this purpose.
When superglue fumes are applied to a surface with fingerprints, a chemical reaction occurs where the fumes adhere to the oily residue left by the fingerprints. This reaction causes the ridges of the fingerprints to appear white against a contrasting background, making them more visible and easier to analyze.
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Ointments are a topical form that is often opaque in appearance. They have a thick and greasy consistency which can make them appear opaque when applied to the skin.
Mordant black indicators are used in complexometric titrations to visually signal the endpoint of the titration. They form colored complexes with metal ions, resulting in a visible color change when all the metal ions have reacted with the titrant. This color change helps in determining the endpoint of the titration accurately.