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Chloroform is immiscible in water, so you can separate chloroform extract from water using liquid-liquid extraction. By adding chloroform to the mixture, the two layers will separate based on their immiscibility. After shaking and allowing the layers to separate, the chloroform layer can be carefully decanted or extracted using a separatory funnel.
To extract benzoic acid from chloroform, first dissolve the benzoic acid in water. Then, add chloroform to the mixture and shake well to allow for the benzoic acid to transfer to the chloroform phase. Finally, separate the two phases and evaporate the chloroform to obtain the benzoic acid.
You can separate water from chloroform by utilizing their difference in density. Since chloroform is denser than water, the mixture can be placed in a separatory funnel. Upon standing, the two liquids will separate into distinct layers, allowing the water to be drained from the bottom.
To separate chloroform or dichloromethane extract from an aqueous solution using a separating funnel, you would add the mixture into the funnel and allow the layers to separate based on their densities. Then, carefully drain the lower aqueous layer while keeping the organic layer in the funnel. Finally, collect the organic layer in a separate container and repeat the process if needed for further purification.
When water and chloroform are mixed, they will form two separate layers due to their different densities. Chloroform is not soluble in water, so they will not mix homogeneously. Chloroform tends to settle as the bottom layer since it is denser than water.
You can quickly confirm which layer is water and which is chloroform by adding a few drops of water to a test tube containing the layers. Water will mix with the layer that is water, causing it to become more transparent. Chloroform will not mix with water and remain as a separate layer.