To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.
salkowski's reaction is carried out using salkowski's reagent (1 ml of 0.5 M FeCl3 + 50 ml of 35 % perchloric acid ). It is used for colorimetric estimation of indole derivates, it produces pink colour. A trace of hydrogen peroxide induces the oxidation reaction which produces the colour and hastening its intensity.
To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.
The principle behind the Salkowski test is to detect the production of indole by bacteria. This test involves the addition of p-dimethylaminocinnamaldehyde reagent to a culture containing tryptophan. If indole is present, a blue color will develop, indicating a positive result.
Tollens' reagent is made by mixing two solutions - silver nitrate and ammonia - right before use because the reaction is sensitive to light, air, and temperature, which can degrade the reagent quickly. Preparing it in advance may lead to a loss of effectiveness, affecting the results of tests. Therefore, it is best to prepare Tollens' reagent fresh when needed for accurate testing.
Ernst Leopold Salkowski died in 1923.
Ernst Leopold Salkowski was born in 1844.
To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.
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salkowski's reaction is carried out using salkowski's reagent (1 ml of 0.5 M FeCl3 + 50 ml of 35 % perchloric acid ). It is used for colorimetric estimation of indole derivates, it produces pink colour. A trace of hydrogen peroxide induces the oxidation reaction which produces the colour and hastening its intensity.
To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.
The principle behind the Salkowski test is to detect the production of indole by bacteria. This test involves the addition of p-dimethylaminocinnamaldehyde reagent to a culture containing tryptophan. If indole is present, a blue color will develop, indicating a positive result.
To prepare Tollens reagent, mix aqueous silver nitrate with ammonia solution until a precipitate forms. Then add sodium hydroxide solution to redissolve the precipitate and form the final reagent. It is used to test for the presence of aldehydes in a reaction.
To prepare Nessler's reagent, dissolve 7g of mercuric iodide and 35g of potassium iodide in distilled water, and then add 100g of potassium hydroxide pellets. Once the solution is clear, dilute it with water to make 1 liter of Nessler's reagent. This reagent is commonly used for detecting ammonia in solutions.
To prepare Seliwanoff's reagent, dissolve resorcinol in concentrated hydrochloric acid, then add a small amount of acetaldehyde. Mix well and dilute with water if needed. This reagent is used for differentiating between aldose and ketose sugars based on the color developed upon heating.
To prepare Kedde's reagent, mix equal volumes of concentrated hydrochloric acid and 5% potassium dichromate solution. The reagent is used to test for the presence of fatty acids in a substance, where a positive test results in the formation of a layer of orange crystals.