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CONVERT TO THE MOLE (0.075 m/l) * (157.67mw) =11.82 g/l

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To make a 75mM Tris HCl buffer solution, you would mix the appropriate amounts of Tris base and hydrochloric acid with water to reach a final concentration of 75mM. It is important to properly calculate the molarity of the stock solutions to achieve the desired concentration accurately and to adjust the pH, if necessary.

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Q: How do you make up 75mM Tris HCL buffer solution?
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How can I make tris acetate?

To make tris acetate, you can mix tris base with acetic acid in a specific ratio and then adjust the pH level to achieve the desired tris acetate buffer solution.


What Volume of 5M HCl to make tris buffer at pH 7.5?

To make a Tris buffer at pH 7.5, you will need to mix Tris base with HCl. To calculate the volume of 5M HCl needed, you will first need to determine the molarity of the Tris buffer solution and then use the Henderson-Hasselbalch equation. The exact volume of 5M HCl required will depend on the amount of Tris base used and the final volume of the buffer solution.


How do you prepare 20 mM tris hcl buffer pH 7.4?

to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris


How do you prepare tris-hcl buffer solution of 0.5M?

To prepare a 0.5M Tris-HCl buffer solution, you would need to dissolve the appropriate amount of Tris base and hydrochloric acid in water. The specific amounts to use can be calculated using the formula c1v1 = c2v2, where c1 is the concentration of the stock solution, v1 is the volume of the stock solution needed, c2 is the desired final concentration, and v2 is the final volume of the solution. Make sure to adjust the pH if necessary using a pH meter or pH meter.


How do you prepare 10mM solution of Tris-HCl?

To prepare a 10mM solution of Tris-HCl, you would weigh out the appropriate amount of Tris-HCl powder using a balance and dissolve it in water to make a final volume of solution. For example, to make 1L of 10mM Tris-HCl solution, you would need to dissolve 0.121g of Tris-HCl in 1L of water.