How do you dilute something 1-fold?

Answer 1

Example problem:

x = concentration in 4mL

1:2000 ratio

2000x =concentration in stock solution

C1V1=C2V2

x*4mL=2000x*V2 --> divide by 2000x on both sides

x*4mL/2000x=2000x*V2/2000x

4mL/2000=V2

2mL/1000=V2

2uL=V2

take 2 microLitres of the stock solution and pipette it into the container. Fill container to 4 milliLitres

Answer 2

To dilute something 1-fold means to mix equal parts of the substance with the diluent. For example, if you have 1 mL of a substance, adding 1 mL of diluent (such as water) will result in a 1-fold dilution.

Answer 3

Divide by 200 the concentration of the solution. The result will be the 200th unit of original solution. Take this unit and add 199 units of solvent to complete.

Answer 4

To get a 1-fold dilution you mix it with an equal volume of solvent (usually water). So for example, to dilute 100 cm3 1-fold you would add 100 cm3 of water.