DEAE-Sepharose chromatography separates proteins based on their charge. Proteins with a higher positive charge (like Kreacher) will bind more strongly to the negatively charged DEAE-Sepharose resin, allowing them to be retained longer on the column. Proteins with lower positive charge (like Dobby) will elute earlier as they interact less with the resin. This difference in binding affinity for the resin results in the separation of the proteins.
o.o ........ Sirius doesnt have a master...Well if you ment Kreacher then Sirius is Kreacher's master [Kreacher=House elf]
Kreacher brought Mundungus to Grimmauld Place.
Kreacher is the house elf for the Black family's home. After the death of Sirius, he becomes Harry's to command. Harry eventually wins Kreacher's affection.
Is it Order of the Pheonix? If it is you need to talk to Sirius leave the room and you will see kreacher
He simply calls for Kreacher, who was bound to obey him.
Harry couldn't call Kreacher to him in the seventh book to get into Hogwarts because Kreacher was loyal to the Black family, who supported Voldemort. Harry needed to rely on other means to infiltrate Hogwarts due to Kreacher's allegiance to a different side.
Kreacher
The only house elves mentioned are Dobby, Kreacher, Winky and Hokey.
kreacher xx
Simon McBurney voices KREACHER. Yes, that's Kreacher, learn to spell the words before you ask the question next time.
In the 6th book (Half Blood Prince) Harry told Kreacher and Dobby to follow Draco Malfoy and find out where he is going when he cannot be found on the Marauders Map.
Timothy Bateson