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An active methylene compound is a molecule that has a methylene group (CH2) adjacent to a carbonyl group (C=O) which can undergo nucleophilic substitution reactions. Examples include compounds like acetylacetone and malonic acid. These compounds are often used in organic synthesis to introduce new functional groups and form carbon-carbon bonds.

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Q: Explain active methylene compound with examples?
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Why methylene blue can act on only dead yeast cells but not on live yeast cells?

Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.


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