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An active methylene compound is a molecule that has a methylene group (CH2) adjacent to a carbonyl group (C=O) which can undergo nucleophilic substitution reactions. Examples include compounds like acetylacetone and malonic acid. These compounds are often used in organic synthesis to introduce new functional groups and form carbon-carbon bonds.

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Q: Explain active methylene compound with examples?
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Process name involved the reaction of yeast and methylene blue?

Methylene blue is a solid, odorless, dark green powder at room temperature. In water this chemical compound turns into a blue solution. When methylene blue is reacted with yeast cells it inhibits the respiration which stops the cells from using hydrogen ions to release energy.


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Methylene blue is used as an indicator of cell respiration because it can change color depending on whether it is in its oxidized or reduced form. When cells are actively respiring, they reduce methylene blue, causing it to change from blue to colorless. This color change is used to detect the presence of active respiration in the cells.


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The hydrogen attached to a saturated carbon atom containing strong electron withdrawing groups in acidic form reacts with base to generate carbanion .such compounds which contain acidic CH2 group are called active methylene compounds . eg:CH3-CO-CH2-COO-C2H5 (ethyl aceto acetate


Why methylene blue can act on only dead yeast cells but not on live yeast cells?

Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.


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