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The Scatchard plot is a graph used in biochemistry to analyze binding data. It helps determine the affinity and number of binding sites between a ligand and a receptor. The plot shows the ratio of bound ligand to free ligand versus the concentration of bound ligand. The slope of the plot provides information about the binding constant, while the x-intercept indicates the total number of binding sites. This analysis can help researchers understand the strength and nature of the interaction between molecules.

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