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This is a method of bacterial transformation; to get bacteria to take up plasmid DNA.

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βˆ™ 13y ago
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βˆ™ 5mo ago

Bacteria are treated with calcium ions and subjected to temperature shock to make them more permeable to foreign DNA, allowing for easier transformation with new genetic material. The calcium ions help destabilize the bacterial cell wall, while the temperature shock creates temporary pores in the membrane, facilitating the uptake of DNA molecules.

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Q: Why are bacteria treated with calcium ions and subjected to temperature shock?
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Bacteria and virus difference?

Bacteria are single-celled organisms with a cell wall and can reproduce on their own, while viruses are not cells but rather genetic material enclosed in a protein coat that must infect a host cell to replicate. Bacteria can be treated with antibiotics, while viruses are typically treated with antiviral medications or vaccines.


Differences between bacteria and virus?

Bacteria are single-celled organisms that can reproduce on their own, while viruses are not cells and rely on a host cell to reproduce. Bacteria are larger in size than viruses and can be treated with antibiotics, whereas viruses are smaller and are typically treated with antiviral medication. Bacteria are living organisms with their own cellular machinery, whereas viruses are considered non-living as they require a host cell to replicate.


Is plastic antibacterial?

Plastic itself is not inherently antibacterial. However, some plastics can be engineered or treated with antibacterial agents to inhibit the growth of bacteria on their surface.


How is a Gram stain used in an experiment involving bacteria?

A Gram stain is used to differentiate bacteria based on their cell wall composition. It can classify bacteria into two main groups, Gram-positive (purple) and Gram-negative (pink/red). This staining technique helps researchers identify and classify bacteria in experiments based on their response to the staining process.


When treated with Gram stain Gram-positive bacteria appear?

purple, due to the retention of the crystal violet dye in the thick peptidoglycan layer in their cell wall.

Related questions

What type of infections can be treated with antibiotics?

Bacteria


Does antibiotics cure bacteria?

Bacteria are treated with antibiotics and viruses are treated with antiviral medications.


How were the Norman slaves treated?

The Norman slaves were treated in an ill manner and not regarded as humans, They were subjected to heavy work and constant whipping by their masters.


How are bacteria treated?

bacteria is treated my medication from a specific doctor, i had ring-worm which is a bacteria and i have medicine (oitment) that i have to use 3 times a day and the ring-worm is almost gone!


What would happen to intracellular calcium levels if a muscle fiber were treated with a calcium channel-blocking drug?

It would increse the intracellular calcium


How is sludge treated?

biologically by anaerobic bacteria


Can a bacteria cell be treated with an antibiotics?

Most can, yes


What skin disorder can be treated with antibiotics?

Any skin disease caused by Bacteria can be treated by antibiotics e.g.boils .Allergies can't be treated by antibiotics .


Did slaves get treated kindly?

No, slaves were not treated kindly. They were often subjected to inhumane and brutal conditions, including physical abuse, forced labor, and separation from their families. Slavery was a system of exploitation and oppression.


How much bacteria does Ivory soap kill?

89.73 percent of all bacteria is killed within the area treated.


When zeolite is treated with hard water sodium ions are exchanged by?

calcium ions present in the hard water, leading to the formation of calcium zeolite.


What happens when carbon dioxide is treated with limewater?

When carbon dioxide is treated with limewater (calcium hydroxide solution), a white precipitate of calcium carbonate forms. This is a chemical reaction that is commonly used to test for the presence of carbon dioxide gas.