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No medium exists for the selective growth of particular Trichoderma species, i.e. unless a pure spore culture is used of T. viride, one won't be able to select for it specifically using growth media. You can however, use a Trichoderma Selective Medium (TSM) to suppress growth of other fungi/bacteria and obtain Trichoderma colonies that can then be differentiated by morphology. Once a pure culture is obtained, subs can then be made onto various media such as potato dextrose agar (PDA), malt extract agar (MEA) on which Trichoderma will happily grow profusely and sporulate. TSM suppresses fungal growth making Trichoderma colonies grow very slowly, so this not a good media to use if sporulation is the objective.
PDA (Potato Dextrose Agar) is commonly used as a selective medium for Trichoderma viride. It provides the necessary nutrients and pH conditions that promote the growth of this fungus while inhibiting the growth of other contaminants.
0 2 g MgSO4 (7H:O) (Unilab), 0 9 g
K:HPO, (Univar), 015 g KCl (Unilab), 10 g
N H4NO3 (Univar),30gD(-(- )glucose anhydrous
(Univar), 0 15 g rose bengal (BDH Chemicals)
and 20 g agar (Merck). These constituents were
added to 950 ml of distilled water and autoclaved
at 121 C for 15 min. The biocidal ingredients
were 0 25 g chloramphenicol (crystallized)
(Boehringer Mannheim), 02 g quintozene
(PCNB. wettable powder, 750 g/kg a.i.) (May Baker), 0-2 g captan (Kaptan 517, wettable
powder, 500 g/kg a.i.) (AECI) and either propamocarb-
hydrochloride (Previcur N-12, solution
concentrate, 722 g/l a.i.) (Schering International)
or metalaxyl (Ridomil 273, granular, 50 g/kg a.i.)
(Ciba Geigy) at 1-2 m) and 1-6 g respectively.
These were mixed in 50 ml of sterilized (autoclaved
at 121 C for 15 min) distilled water and
added to the autoclaved basal medium. Thus,
except for propamocarb or metalaxyl (chosen for
anti-oomycete activity) replacing fenaminosulf,
this medium was identical to that of Elad & Chet
(1983).
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Is pseudomona aeruginosa grow in xld agar?
Pseudomonas aeruginosa typically does not grow well on Xylose Lysine Deoxycholate (XLD) agar, as this medium is selective for Enterobacteriaceae. Pseudomonas species are usually inhibited by the selective agents in XLD agar. It is better to use selective media like Pseudomonas agar for the isolation and differentiation of Pseudomonas aeruginosa.
Is Sodium lactate agar selective?
Sodium lactate agar is not inherently selective. It can be used as a general growth medium for a wide range of microorganisms due to its nutrient content. However, it can be modified by adding selective agents to make it selective for specific organisms.
Why is Sabouraud's dextrose agar a selective medium?
Sabouraud's dextrose agar is a selective medium because its low pH inhibits the growth of most bacteria while promoting the growth of fungi and yeast. Additionally, the high sugar content promotes the growth of fungi and suppresses the growth of bacteria.
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
The bacteria containing the plasmid with the integrated eukaryotic gene would grow in a selective medium that supports the growth of bacteria carrying the plasmid. This medium would typically contain an antibiotic or a specific nutrient that selects for bacteria with the plasmid.
Will s aureus grow on a crystal violet agar plate?
Staphylococcus aureus can grow on crystal violet agar plates as crystal violet agar is a selective medium that inhibits the growth of Gram-negative bacteria and allows the growth of Gram-positive bacteria like S. aureus.
Macconkey agar is a selective medium and it is also a defined or a undefined medium so why is that a formulation desirable?
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
Is pseudomona aeruginosa grow in xld agar?
Pseudomonas aeruginosa typically does not grow well on Xylose Lysine Deoxycholate (XLD) agar, as this medium is selective for Enterobacteriaceae. Pseudomonas species are usually inhibited by the selective agents in XLD agar. It is better to use selective media like Pseudomonas agar for the isolation and differentiation of Pseudomonas aeruginosa.
Is Sodium lactate agar selective?
Sodium lactate agar is not inherently selective. It can be used as a general growth medium for a wide range of microorganisms due to its nutrient content. However, it can be modified by adding selective agents to make it selective for specific organisms.
Why isolation of a pure culture on selective medium may not be successful?
Isolation of a pure culture on a selective medium may not be successful due to factors such as contamination from other organisms, genetic variability within the target organism, or difficulties in selecting appropriate conditions for growth. Additionally, some organisms may not grow well under the specified selective conditions, leading to a lack of colony formation.
Will p. aeruginosa grow on a blood agar media?
Yes, Pseudomonas aeruginosa can grow on blood agar media because it is a non-selective medium that provides essential nutrients for bacterial growth. However, its growth on blood agar may not be as robust as on selective media designed specifically for its isolation and identification.
Why is Sabouraud's dextrose agar a selective medium?
Sabouraud's dextrose agar is a selective medium because its low pH inhibits the growth of most bacteria while promoting the growth of fungi and yeast. Additionally, the high sugar content promotes the growth of fungi and suppresses the growth of bacteria.
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
The bacteria containing the plasmid with the integrated eukaryotic gene would grow in a selective medium that supports the growth of bacteria carrying the plasmid. This medium would typically contain an antibiotic or a specific nutrient that selects for bacteria with the plasmid.
What type of culture medium is agar beef heart and human blood?
Agar beef heart and human blood is a selective culture medium that is specific for detecting certain bacteria, such as fastidious organisms that require nutrients found in blood and heart tissue to grow. This medium is commonly used for isolating and identifying pathogens like Haemophilus influenzae.
List two methods for obtaining isolated colonies.?
Use a "streak plate" or a "spread plate". Mediums that allow some organisms to grow and not others can also be used. These types of mediums are called Selective medium.
Will s aureus grow on a crystal violet agar plate?
Staphylococcus aureus can grow on crystal violet agar plates as crystal violet agar is a selective medium that inhibits the growth of Gram-negative bacteria and allows the growth of Gram-positive bacteria like S. aureus.
How tall will a twisty baby locust grow?
A twisty baby locust tree can grow to be around 6-9 feet tall, depending on environmental conditions and care. It is considered a small to medium-sized tree and can be a unique and attractive addition to landscaping.
Where does soil grow?
Potting soil does not grow. It is not a plant, but a medium in which to grow plants.
