0
Add your answer:
Earn +20 pts
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Which of the following removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
Why rna primer is used instead of dna primer?
RNA primers are used in DNA replication because RNA primers are easier to synthesize compared to DNA primers. RNA primers are complementary to the DNA template strand and provide a starting point for DNA polymerase to begin replication. Once the new DNA strand is synthesized, the RNA primer is removed and replaced with DNA.
For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
For DNA polymerase to link nucleotides together, the first nucleotide must be attached to a primer, which is a short segment of RNA or DNA that provides a free 3' hydroxyl group for the DNA polymerase to start adding nucleotides. DNA polymerase can only extend nucleotides from an existing primer or strand, using it as a template for complementary base pairing.
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
What is the function of primer in PCR?
it synthesizes a single RNA primer at the 5' end of the leading end.
What is a RNA primer removed by?
A RNA primer in DNA replication is removed by an enzyme called DNA polymerase I in prokaryotes and DNA polymerase δ in eukaryotes. These enzymes have exonuclease activity that can remove RNA primers and replace them with DNA nucleotides.
What does DNA primase create?
DNA primase creates short RNA sequences called primers that provide a starting point for DNA replication. These primers are later used by DNA polymerase to synthesize new DNA strands.
What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?
A primer molecule is required for DNA polymerase to initiate the addition of nucleotides. This primer provides a starting point for DNA polymerase to begin adding nucleotides in the correct sequence. Once the primer is in place, DNA polymerase can add nucleotides complementary to the template strand.
What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Which of the following removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
In polymerase chain reaction how many kinds of primer are used?
In polymerase chain reaction (PCR), two types of primers are used: a forward primer and a reverse primer. These short DNA sequences are specific to the target DNA region to be amplified and serve as starting points for DNA synthesis by the DNA polymerase enzyme.
Which of the following is needed in the process of DNA replication?
The ingredients needed for DNA replication include a short oligonucleotide primer and dNTPS. It also needs DNA polymerase and different transcription and translation factors.
For DNA polymerase to link nucleotides together as new strands of DNA the first nucleotide must be attached to what because DNA polymerase can only add nucleotides to a pre exsisting strand?
The first nucleotide must be attached to a short RNA primer to provide a free 3' hydroxyl group for DNA polymerase to extend from. DNA polymerase starts adding nucleotides to this RNA primer to begin DNA replication.
Why rna primer is used instead of dna primer?
RNA primers are used in DNA replication because RNA primers are easier to synthesize compared to DNA primers. RNA primers are complementary to the DNA template strand and provide a starting point for DNA polymerase to begin replication. Once the new DNA strand is synthesized, the RNA primer is removed and replaced with DNA.
For DNA polymerase to link nucleotide together as new strands of DNA the first nucleotide must be attached to a because DNA polymerase can only add nucleotides to a pre-existing strand?
For DNA polymerase to link nucleotides together, the first nucleotide must be attached to a primer, which is a short segment of RNA or DNA that provides a free 3' hydroxyl group for the DNA polymerase to start adding nucleotides. DNA polymerase can only extend nucleotides from an existing primer or strand, using it as a template for complementary base pairing.
