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your sample can destroy the bacteria and it will appear shapeless or enlarged when you overheat the bacterial smear

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15y ago
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5mo ago

Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.

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Q: What would be the result of overheating the bacterial smear?
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How would you define a properly prepared bacterial smear?

A properly prepared bacterial smear involves spreading a thin, even layer of bacterial sample on a clean microscope slide. This is usually achieved by using a sterile loop or swab. The slide is then heat-fixed to kill the bacteria and adhere them to the slide for staining and microscopic examination.


What are the advantage and disadvantage of heat fixing?

Advantages: It helps adhere bacterial cells to the slide, preventing them from washing away during staining. Also, it kills the bacteria, making them safe to handle and study under the microscope. Disadvantages: Heat fixing can distort the morphology of the bacterial cells, affecting the accuracy of the staining results. Overheating can also cause cell lysis, leading to inaccurate interpretation of the specimen.


What is the procedural difference between using broth and agar as a source of bacteria for preparing a smear?

When using broth as a bacterial source for preparing a smear, you would typically take a small amount of broth and place it on a slide for staining. With agar, you would use a loop or swab to pick up bacteria from a colony growing on an agar plate, then transfer it to a slide. This is because broth contains bacteria in suspension while agar contains bacteria in colonies.


What would not be found in a bacterial cell?

A nucleus, mitochondria, or endoplasmic reticulum would not be found in a bacterial cell. Bacterial cells are prokaryotic and lack membrane-bound organelles typically found in eukaryotic cells.


Does the human body have more bacterial or human cells?

it has more human cells actually the human body has more bacterial cells. Although it may seem more likely that the human body would have more human cells than bacterial cells. -Vasillisa

Related questions

Why would a pap smear take 4 months for the result to come back?

A pap smear does not take four months for results. Contact your health care provider directly to find out why the result is not in.


How would you define a properly prepared bacterial smear?

A properly prepared bacterial smear involves spreading a thin, even layer of bacterial sample on a clean microscope slide. This is usually achieved by using a sterile loop or swab. The slide is then heat-fixed to kill the bacteria and adhere them to the slide for staining and microscopic examination.


Does the NuvaRing need to be removed for a pap smear?

No, it does not need to be removed for a Pap smear. The annual gyn exam and testing can be done with the Nuva Ring in place. Not that I know of. I have had a pap smear done with it in. It may just be the practitioner's preference. If so, then she/he can probably remove and place it back immediately after the exam. But I would leave it in and inform my practitioner.


Can you heat fix blood smear before staining for bacterial examination?

You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.


Can check up pap smear before surgery ovarian cyst?

Yes, you can get a pap smear before surgery for an ovarian cyst. It would be wise to do so, so that any abnormal result might be evaluated at the same time as the surgery.


During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?

Without heat fixing, the bacteria on the slide will not adhere properly, leading to poor attachment to the slide during staining. This may result in uneven staining, leading to difficulty in visualizing the bacterial cells clearly under the microscope. Proper heat fixing ensures that the bacteria are securely attached to the slide, allowing for better staining and clearer observation under the microscope.


Does zoom whitening cause perioral dermatitis?

No, perioral dermatitis is a result of a bacterial infection and would have nothing to do with zoom whitening.


Would obtaining a PAP smear be a surgical or medical asepsis?

Obtaining a pap smear uses medical asepsis. There is no break in skin when getting a pap smear.


What is meant by heat fixing a bacterial smear?

to allow the excess water to dry out before heating. if heated right after, the water would cause the smear to overheat and denature some features in the stain. and those features would no longer be seen


Is latex condom safe in case of inflammation in pap smear test?

Using condoms would be a great strategy if you had inflammation on your pap result. Condoms can lower the chances of infection spreading.


Are chronic arthritis usually result from bacterial invasion?

My mother has Alzheimer's and Arthritis in her knee and hip. She is 83, what can i give her, that would help her walk better.


Can your water break while getting a pap smear done?

Sure, it's possible for your water to break during a pap smear. There is nothing about the pap smear process that would cause your water to break.