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Crystal violet is the primary stain in the Gram's stain procedure, used to color all bacteria cells purple. This helps differentiate between Gram-positive bacteria (which retain the violet color) and Gram-negative bacteria (which lose the violet color when decolorized with alcohol).
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What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
What color does the gram positive stain?
It will hold the primary stain of violet.
Can grams iodine be used before primary stain?
Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives. Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives.
What is mordant used in the gram staining procedure and its function?
Iodine is the mordant used in the gram staining procedure. Its function is to complex with the crystal violet dye, forming large crystal iodide molecules that get trapped within the thick peptidoglycan layer of gram-positive bacteria. This helps to stabilize the crystal violet within the gram-positive bacteria, making them appear purple under the microscope.
Why would gram negative bacteria appear purple after gram stain?
Gram-negative bacteria appear pink after a Gram stain procedure because they have a thinner layer of peptidoglycan in their cell walls, which doesn't retain the crystal violet stain used in the process. The counterstain, usually safranin, then colors the bacteria pink.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
How many grams are in a 50000 kg crystal?
There are 50,000,000 grams in a 50,000 kg crystal. This is because there are 1,000 grams in 1 kilogram.
What does half a ball of crystal meth weigh?
Half a ball of crystal meth typically weighs around 1.75 grams to 2 grams, depending on the quality and density of the crystal.
How much do a ounce Crystal meth weigh?
An ounce of crystal meth weighs approximately 28 grams.
How much did a small crystal weigh?
On average, a small crystal weighs between 2-10 grams depending on the type and size of the crystal.
What color does the gram positive stain?
It will hold the primary stain of violet.
How much does a half ounce of crystal meth weigh?
A half ounce of crystal meth weighs approximately 14 grams.
What does an eight ball of crystal meth weigh?
The term "eight ball" refers to a quantity of cocaine or crystal meth that is equal to an eighth of an ounce, which is equivalent to 3.5 grams.
How many grams are there in a pound of crystal meth?
There are approximately 453.592 grams in a pound of crystal meth.
Can grams iodine be used before primary stain?
Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives. Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives.
How much does a half ounce of crystal meth weigh on a digital scale?
common sence there is 28 grams in an ounce
