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A PCR case typically refers to a case in which a polymerase chain reaction (PCR) test is used to detect the presence of a specific genetic material, such as a virus or bacteria. PCR testing is a common method for diagnosing Infectious Diseases like COVID-19.
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What is the defference between Real-time PCR and reverse transcriptase PCR?
Real-time PCR is a technique used for quantifying DNA in real-time during the PCR process, while reverse transcriptase PCR (RT-PCR) is used to detect RNA by first converting it to complementary DNA (cDNA) using reverse transcriptase enzyme before proceeding with PCR amplification. Real-time PCR allows for monitoring the amplification process as it occurs, while RT-PCR is specifically used for analyzing RNA levels.
What is difference between Qualitative PCR and Quatitative PCR?
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
How you calculate nested pcr product size?
To calculate the size of the nested PCR product, you would first determine the size of the first PCR product by adding the sizes of the primers and the DNA template. Then use the first PCR product size as the template size for the second PCR reaction, adding the sizes of the second set of primers to estimate the final nested PCR product size. Keep in mind that any additional flanking regions may also contribute to the final product size.
How long has real time pcr been around?
Real-time PCR, also known as quantitative PCR (qPCR), has been around since the mid-1990s. It gained popularity for its ability to monitor the amplification of DNA during the PCR process in real time, providing quantitative data on DNA or RNA targets.
Why do you use a negative control in PCR?
A negative control is used in PCR to ensure that there is no contamination in the reaction, which could lead to false positive results. It contains all the PCR components except the template DNA, so any amplification detected in the negative control would indicate contamination.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is pcr and types of pcr?
PCR (polymerase chain reaction) is a molecular biology technique used to amplify a specific segment of DNA. There are various types of PCR, including quantitative PCR (qPCR) for quantification of DNA, reverse transcription PCR (RT-PCR) to amplify RNA, nested PCR for increased specificity, and digital PCR for absolute quantification of nucleic acids.
What are the differences between conventional pcr andreal time pcr?
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
What is the defference between Real-time PCR and reverse transcriptase PCR?
Real-time PCR is a technique used for quantifying DNA in real-time during the PCR process, while reverse transcriptase PCR (RT-PCR) is used to detect RNA by first converting it to complementary DNA (cDNA) using reverse transcriptase enzyme before proceeding with PCR amplification. Real-time PCR allows for monitoring the amplification process as it occurs, while RT-PCR is specifically used for analyzing RNA levels.
What is difference between Qualitative PCR and Quatitative PCR?
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
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What is the difference between simplex and multiplex pcr?
what is the difference between PCR simplex and multiplex
What is the role of pcr in laser toner?
Pcr serves to transfer an electric charge to the surface of photo conductor drum located in toner cartrige, the pcr is in contact with opc drum as drum turns,any loosr deposits on pcr transfarred to the drum
Types of pcr and their significance?
there are; 1. RT PCR - helps in making complementary DNA with the help of mRNA. 2.anchored PCR - helps in making the DNA whose sequence is unknown.
What is the function of tris hcl in PCR buffer?
Tris HCl in PCR buffer helps to maintain a stable pH during the PCR reaction. It acts as a buffering agent, preventing pH changes that could affect the efficiency of the DNA amplification process. This helps to optimize the conditions for the PCR reaction to occur successfully.
What is the purpose of the mineral oil used for PCR when the termocycler does not have a hot lid?
To prevent evaporation of PCR products.
