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In pglo experiments, a control plate is a plate that contains bacterial cells that have not been genetically modified with the plasmid that carries the GFP gene and the resistance gene. This plate is used as a comparison to observe any changes or effects caused by the presence of the plasmid in the experimental plates.
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Why use arabinose in the plate in pglo experiments?
Arabinose is used in the plate in pglo experiments to induce the expression of the Green Fluorescent Protein (GFP) gene. The presence of arabinose activates the araC promoter, allowing for the transcription and translation of the GFP gene, which results in the production of green fluorescent protein in the bacteria. This fluorescence helps researchers visualize and track the transformation of the bacteria with the desired gene.
What is the small ring of DNA in bacteria?
The small ring of DNA in bacteria is called a plasmid. Plasmids are separate from the bacterial chromosome and can contain genes that provide advantages, such as antibiotic resistance, for the bacteria. Plasmids can be transferred between bacteria, allowing for the spread of these advantageous genes.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
Why use arabinose in the plate in pglo experiments?
Arabinose is used in the plate in pglo experiments to induce the expression of the Green Fluorescent Protein (GFP) gene. The presence of arabinose activates the araC promoter, allowing for the transcription and translation of the GFP gene, which results in the production of green fluorescent protein in the bacteria. This fluorescence helps researchers visualize and track the transformation of the bacteria with the desired gene.
On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?
You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.
What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose was to observe the differences in fluorescence levels between the pGLO solution under normal conditions and when exposed to UV light. This allowed for visual confirmation of the presence and activation of the GFP gene in the pGLO solution when exposed to UV light.
What traits of the E. coli were not altered in pGLO experiment?
Colony shape and color
Can you tell if these bcteria are ampicillin resistant by looking at them on the LB plate?
No, you cannot determine if bacteria are ampicillin resistant just by looking at them on an LB plate. Ampicillin resistance typically requires a specific test, like a disk diffusion assay or a genetic analysis, to confirm resistance. Visual observation alone is not sufficient.
Bio ap ap pglo transformation?
What kind of question is that? I mean really genius. I mean all your saying is the labs name and the class...that is not a question.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
What are the two possible sources of fluorescence within an Ecol colony when exposed to UV light?
The two possible sources of fluorescence within an E. coli colony when exposed to UV light are autofluorescence of the colony itself, caused by endogenous fluorophores present in the cells, and expression of a fluorescent protein, such as green fluorescent protein (GFP), as a result of genetic manipulation.
What is the small ring of DNA in bacteria?
The small ring of DNA in bacteria is called a plasmid. Plasmids are separate from the bacterial chromosome and can contain genes that provide advantages, such as antibiotic resistance, for the bacteria. Plasmids can be transferred between bacteria, allowing for the spread of these advantageous genes.
