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restriction enzyme site

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13y ago
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4mo ago

HinF1 site is a specific sequence of base pairs (5'-GANTC-3') on a DNA molecule that is recognized and cut by the restriction enzyme HinF1. When HinF1 cleaves the DNA at its site, it generates specific DNA fragments with defined ends that can be useful for molecular Biology applications such as DNA analysis or cloning.

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Q: What is HinF1 site in DNA sample?
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When DNA profiling is carried out the second DNA sample the one not collected at the crime scene is called the?

mitochondrial sample


How many restriction enzymes are needed to positively identify a sample of DNA?

Multiple restriction enzymes are often needed to positively identify a sample of DNA due to the complexity and size of the genome. Using a combination of restriction enzymes can provide a unique digestion pattern that serves as a distinctive "fingerprint" for a specific DNA sample.


Can a sample of blood from a piece of clothing be use for DNA testing?

Yes, a sample of blood from a piece of clothing can be used for DNA testing. The DNA extracted from the blood can be compared to known DNA samples to help identify a person or provide information about their biological relationships.


What is the principle behind Quantification of DNA by reading its optical density at 260nm and 280nm?

The principle behind quantifying DNA by measuring its optical density at 260nm and 280nm is based on the fact that DNA absorbs light at these specific wavelengths. The ratio of the absorbance at 260nm to 280nm is used to assess the purity of the DNA sample, with a 260/280 ratio of around 1.8 considered indicative of pure DNA. By comparing the absorbance values at these two wavelengths, scientists can estimate the concentration and purity of DNA in a sample.


What is a DNA restriction site?

A DNA restriction site is a specific short nucleotide sequence recognized by a restriction enzyme, which cuts the DNA at that site. These sites are often palindromic, meaning the sequence reads the same forwards and backwards. Restriction sites are commonly used in molecular biology for DNA manipulation and analysis.

Related questions

When DNA profiling is carried out the second DNA sample the one not collected at the crime scene is called the?

mitochondrial sample


What is used to create a large sample of DNA for testing for a small sample of DNA?

A technique called polymerase chain reaction (PCR) is used to create a large sample of DNA from a small sample. PCR amplifies specific regions of DNA by making millions of copies, allowing for further analysis and testing on the amplified DNA.


Should the police get your DNA sample?

In most of the countries the police are not allowed to get your DNA sample without the permission of the court.


How do you find whether given sample is DNA or RNA?

A spectrophotometer can be used to know if a sample is DNA or RNA. DNA has an absorbance maximaat 260nm, whereas RNA has an absorbance maxima at 280nm. By looking at which one of these two wavelengths the sample is more excited, one can determine if the sample is DNA or RNA.


Who owns a sample of DNA?

Everyone? Except people who are imaginary, no DNA for them.


Can a scientist tell from DNA result alone what type of DNA sample were used in the test i.e. is it from blood or semen sample?

Yes, because each DNA is unique and and the scientist can just match the DNA up with andother DNA.


What equipment do they use analyze sample?

what equipment do DNA use to analyzle sample?


What is a sentence of the word sample?

A sample of the girl's DNA led to the breakthrough in the case.


How can DNA solve crimes?

It can be used to identify the criminals easily if the criminal's hair sample or blood sample is collected and comparing it with the DNA of the suspects


What is the method that creates a large sample of DNA for testing from a small sample of DNA?

That would probably be polymerase chain reaction or PCR for short.


Can you get a DNA from me from a girls breast?

can the police get a dna sample from me if i had touched a girls breast


What process could a what forensic scientist use to analyze a DNA sample from a crime scene to identify a criminal?

A forensic scientist would use a method like PCR (Polymerase Chain Reaction) to amplify the DNA sample, creating more copies for analysis. They would then use DNA sequencing techniques to compare the sample to a suspect's DNA profile or a DNA database to identify a match. This process helps determine the source of the DNA sample and link a suspect to the crime scene.